LOCUS       sequence-155885-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Liberti WA 3rd, Markowitz JE, Perkins LN, Liberti DC, Leman DP, 
            Guitchounts G, Velho T, Kotton DN, Lois C, Gardner TJ
  TITLE     Unstable neurons underlie a stable learned behavior.
  JOURNAL   Nat Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405.
  PUBMED    27723744
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405."
COMMENT     Sequence Label: pHAGE-RSV-tdTomato-2A-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(176..232)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      244..253
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(260..274,275..283))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(287..319)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(323..340)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(350..403)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(499..522)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(697..716)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 actgcacgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
       61 tctgctggta gtggtaggcg agctgcacgc cgccgtcctc gatgttgtgg cggatcttga
      121 agttcgcctt gatgccgttc ttctgcttgt cggccttgat atagacgttc tcgagtgagc
      181 tcagccgacc tatagctctg actgcgtgac ctgtcttatt ccacttacga cgtgatgagt
      241 cgaccatggt ggcgagatcc ttatcgtcat cgtcgtacag atcccgaccc atttgctgtc
      301 caccagtcat gctagccata ccatgatgat gatgatgatg agaacccata gggccgggat
      361 tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctctccgctg ccagacttgt
      421 acagctcgtc catgccgtac aggaacaggt ggtggcggcc ctcggagcgc tcgtactgtt
      481 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag cttggtgtcc acgtagtagt
      541 agccgggcag ttgcacgggc ttcttggcca tgtagatggt cttgaactcc accaggtagt
      601 ggccgccgtc cttcagcttc agggcctggt ggatctcgcc cttcagcacg ccgtcgcggg
      661 ggtacaggcg ctcggtggag gcctcccagc ccatggtctt cttctgcatt acggggccgt
      721 cgggggggaa gttggtgccg cgcatcttca ccttgtagat cagcgtgccg tcctgcaggg
      781 aggagtcctg ggtcacggtc accagaccgc cgtcctcgaa gttcatcacg cgctcc
//
