LOCUS       sequence-155888-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for homology-directed repair. 
            Contains the visible marker 3xP3-DsRed flanked by piggyBac termini..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  TITLE     pHD-pBac-DsRed
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScarlessHD-DsRed
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   259..293
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(589..608)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(614..633)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        675..766
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
       61 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
      121 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
      181 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
      241 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaagga
      301 agagccgtcg ctcttccccg ggaaaagaag accatatacg tctcctatag gtctcgtata
      361 ctgcttccta tacgacatca ccgatgggga acacgaagcg atccagcccc ccttattagc
      421 ccgggcgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc
      481 cctactctcg catggggagt ccccacacta ccatcggcgc tacggcgttt cacttctgag
      541 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaaca aggggtgtta
      601 tgagccatat tcaggtataa atgggctcgc gataatgttc agaattggtt aattggttgt
      661 aacactgacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagaatatga gtattcaaca
      781 tttccgtgtc gcccttattc cct
//
