LOCUS       sequence-155890-        9794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains pBAD-CusR (response regulator) and 
            pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy 
            tracking experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH003
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             809..838
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             854..871
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(927..944)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1097..1143
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1187..1207)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1187..1205)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1226..1242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1226..1242)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1239..1261)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1250..1266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1274..1280,1281..1298,1299..1304))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1319..1340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(1745..2539)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1856..1875)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2466..2485
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      4019..4788
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             4789..5451
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     6143..6165
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6158..6174
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6184..6203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6184..6202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      6243..6329
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6424..6510
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6605..6691
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6786..6872
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7025..7903)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        7930..8214
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     8137..8156
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      9111..9182
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9198..9225
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             9729..9794
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gcaactagcg gcatggttag taaaggagaa gaacttttca ctggagttgt cccaatttta
       61 gttgaactag atggcgacgt gaacggtcat aagttcagtg tctccggcga aggtgagggt
      121 gatgcaacgt acggtaagtt aactttgaag ttaatatgta caaccggcaa gctgcctgtt
      181 ccctggccta ccctggtgac aacgttaggt tatgggttga tgtgctttgc tagataccca
      241 gatcacatga aaaggcatga cttctttaaa tctgcaatgc cagaaggtta cgtccaagaa
      301 cgtactattt tctttaaaga tgacggtaat tataaaacta gggctgaagt taaattcgaa
      361 ggtgacacac ttgtaaatcg aatagagtta aaggggattg atttcaaaga ggatggtaat
      421 attctaggcc ataaacttga atataactat aattcacaca acgtttacat taccgccgac
      481 aagcagaaga atggaatcaa agccaatttt aagattagac acaatattga ggatggtgga
      541 gtacagcttg ctgatcatta ccaacaaaat accccgatcg gtgatggacc agttttgcta
      601 cccgataacc attatctgtc ctatcaaagc aaattgtcaa aagatcctaa cgaaaaaaga
      661 gaccacatgg tactcttgga atttgtaaca gctgctggga ttacacatgg catggatgaa
      721 ctatacaaag gttctggtac cgcagcagca aacgacgaaa actacgctgc agcagtttaa
      781 taatacaaat agtgagaagc ttgggcccga acaaaaactc atctcagaag aggatctgaa
      841 tagcgccgtc gaccatcatc atcatcatca ttgagtttaa acggtctcca gcttggctgt
      901 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
      961 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1021 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1081 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1141 tatctgttgt ttgtcggtga actaatttct agaccagctt ttgttccctt tagtgagggt
     1201 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1261 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1321 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1381 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1441 ggcgcatgca taaaaactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1501 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1561 atttgcccat gggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     1621 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     1681 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
     1741 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     1801 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     1861 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     1921 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     1981 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     2041 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     2101 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     2161 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     2221 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     2281 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     2341 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     2401 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     2461 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     2521 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gattttgatc
     2581 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     2641 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
     2701 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
     2761 cgttttccct tgtccagata gcccagtagc tgacattcat cccaggtggc acttttcggg
     2821 gaaatgtgcg cgcccgcgtt cctgctggcg ctgggcctgt ttctggcgct ggacttcccg
     2881 ctgttccgtc agcagctttt cgcccacggc cttgatgatc gcggcggcct tggcctgcat
     2941 atcccgattc aacggcccca gggcgtccag aacgggcttc aggcgctccc gaaggtctcg
     3001 ggccgtctct tgggcttgat cggccttctt gcgcatctca cgcgctcctg cggcggcctg
     3061 tagggcaggc tcatacccct gccgaaccgc ttttgtcagc cggtcggcca cggcttccgg
     3121 cgtctcaacg cgctttgaga ttcccagctt ttcggccaat ccctgcggtg cataggcgcg
     3181 tggctcgacc gcttgcgggc tgatggtgac gtggcccact ggtggccgct ccagggcctc
     3241 gtagaacgcc tgaatgcgcg tgtgacgtgc cttgctgccc tcgatgcccc gttgcagccc
     3301 tagatcggcc acagcggccg caaacgtggt ctggtcgcgg gtcatctgcg ctttgttgcc
     3361 gatgaactcc ttggccgaca gcctgccgtc ctgcgtcagc ggcaccacga acgcggtcat
     3421 gtgcgggctg gtttcgtcac ggtggatgct ggccgtcacg atgcgatccg ccccgtactt
     3481 gtccgccagc cacttgtgcg ccttctcgaa gaacgccgcc tgctgttctt ggctggccga
     3541 cttccaccat tccgggctgg ccgtcatgac gtactcgacc gccaacacag cgtccttgcg
     3601 ccgcttctct ggcagcaact cgcgcagtcg gcccatcgct tcatcggtgc tgctggccgc
     3661 ccagtgctcg ttctctggcg tcctgctggc gtcagcgttg ggcgtctcgc gctcgcggta
     3721 ggcgtgcttg agactggccg ccacgttgcc cattttcgcc agcttcttgc atcgcatgat
     3781 cgcgtatgcc gccatgcctg cccctccctt ttggtgtcca accggctcga cgggggcagc
     3841 gcaaggcggt gcctccggcg ggccactcaa tgcttgagta tactcactag actttgcttc
     3901 gcaaagtcgt gaccgcctac ggcggctgcg gcgccctacg ggcttgctct ccgggcttcg
     3961 ccctgcgcgg tcgctgcgct cccttgccag cccgtggata tgtggacgat ggccgcgagc
     4021 ggccaccggc tggctcgctt cgctcggccc gtggacaacc ctgctggaca agctgatgga
     4081 caggctgcgc ctgcccacga gcttgaccac agggattgcc caccggctac ccagccttcg
     4141 accacatacc caccggctcc aactgcgcgg cctgcggcct tgccccatca atttttttaa
     4201 ttttctctgg ggaaaagcct ccggcctgcg gcctgcgcgc ttcgcttgcc ggttggacac
     4261 caagtggaag gcgggtcaag gctcgcgcag cgaccgcgca gcggcttggc cttgacgcgc
     4321 ctggaacgac ccaagcctat gcgagtgggg gcagtcgaag gcgaagcccg cccgcctgcc
     4381 ccccgagcct cacggcggcg agtgcggggg ttccaagggg gcagcgccac cttgggcaag
     4441 gccgaaggcc gcgcagtcga tcaacaagcc ccggaggggc cactttttgc cggaggggga
     4501 gccgcgccga aggcgtgggg gaaccccgca ggggtgccct tctttgggca ccaaagaact
     4561 agatataggg cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca
     4621 acagctcatt gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat
     4681 tgactgccac ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat
     4741 tgcgcatggt gccgcaaccg tgcggcaccc taccgcatgg agataagcat ggccacgcag
     4801 tccagagaaa tcggcattca agccaagaac aagcccggtc actgggtgca aacggaacgc
     4861 aaagcgcatg aggcgtgggc cgggcttatt gcgaggaaac ccacggcggc aatgctgctg
     4921 catcacctcg tggcgcagat gggccaccag aacgccgtgg tggtcagcca gaagacactt
     4981 tccaagctca tcggacgttc tttgcggacg gtccaatacg cagtcaagga cttggtggcc
     5041 gagcgctgga tctccgtcgt gaagctcaac ggccccggca ccgtgtcggc ctacgtggtc
     5101 aatgaccgcg tggcgtgggg ccagccccgc gaccagttgc gcctgtcggt gttcagtgcc
     5161 gccgtggtgg ttgatcacga cgaccaggac gaatcgctgt tggggcatgg cgacctgcgc
     5221 cgcatcccga ccctgtatcc gggcgagcag caactaccga ccggccccgg cgaggagccg
     5281 cccagccagc ccggcattcc gggcatggaa ccagacctgc cagccttgac cgaaacggag
     5341 gaatgggaac ggcgcgggca gcagcgcctg ccgatgcccg atgagccgtg ttttctggac
     5401 gatggcgagc cgttggagcc gccgacacgg gtcacgctgc cgcgccggta gcacttgggt
     5461 tgcgcagcaa cccgtaagtg cgctgttcca gactatcggc tgtagccgcc tcgccgccct
     5521 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     5581 aatggaagcc ggcggcacct cgctaacgga ttcaccgttt ttatcaggct ctgggaggca
     5641 gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct
     5701 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     5761 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     5821 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt
     5881 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagtc
     5941 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6001 attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6061 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     6121 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     6181 gcgtaatacg actcactata gggcgaattg gagctggggc cgcaattccc aattccaggc
     6241 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     6301 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     6361 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat taattccagg
     6421 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     6481 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     6541 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattccag
     6601 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     6661 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
     6721 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga attaattcca
     6781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     6841 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     6901 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg aattggggat
     6961 cggaagctga attcactagt ctatgctact ccatcgagcc gtcaattgtc tgattcgtta
     7021 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
     7081 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
     7141 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
     7201 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
     7261 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
     7321 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
     7381 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
     7441 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
     7501 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     7561 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     7621 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     7681 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     7741 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     7801 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     7861 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
     7921 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
     7981 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
     8041 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
     8101 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
     8161 gattagcgga tcttacctga cgctttttat cgcaactctc tactgtttct ccatacccgc
     8221 tagcggtacc tcacgcgata cagatctatg aaactgttga ttgtcgaaga tgaaaagaaa
     8281 accggagaat acttgaccaa agggttaacc gaagccggtt ttgtggtcga tttggccgac
     8341 aacgggctga atggctacca tctggcgatg accggtgatt atgatctgat aatcctcgat
     8401 attatgctgc cggacgtgaa cggctgggat atcgtgcgca tgttacgctc cgccaataaa
     8461 gggatgccga ttctgttgct taccgcgctt ggcaccattg aacatcgcgt caaggggctg
     8521 gagttggggg cagatgacta cctggtgaag ccattcgctt ttgctgaact gctggcgcgg
     8581 gtgcgcacat tactgcggcg cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc
     8641 gatttgatgg tcgatctcgt cagccgcaaa gtcacccgca gcggcacgcg catcactttg
     8701 accagtaaag agtttactct gctggagttc ttccttcgcc atcagggcga agtgctgccc
     8761 cgctcgctta tcgcctcgca ggtatgggac atgaattttg acagcgatac caatgctatt
     8821 gatgtggcgg tgaagcggct gcgcggcaaa atcgacaacg actttgagcc gaagctaatt
     8881 cagaccgtgc gcggcgtggg ttacatgctt gaggtgccgg atggtcaggg atctgggtcc
     8941 ggcagcggtg gatctctgga aatccgcgca gcgtttcttg aaaaagaaaa caccgcactg
     9001 cgtacccgcg cggccgaact ccgtaaacgt gtgggccggt gccgcaacat tgtatccaaa
     9061 tacgaaaccc gatacggccc gctttaagga tcctgactcg agccaggcat caaataaaac
     9121 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     9181 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat accgcggcct
     9241 aggattggca aaatgacaat tttgtcattt ttctgtcacc ggaaaatcag agcctggcga
     9301 gtaaagttgg cggcataaaa tcaccagaaa ttatgagctc atacattcgt aagaaacggg
     9361 cgcaggagga ttagatggca gagtatgtgc gggccctgtt tgactttaat gggaatgatg
     9421 aagaagacct tccctttaag aaaggagaca tcctgagaat ccgggataag cctgaagagc
     9481 agtggtggaa tgcagaggac agcgaaggaa agagggggat gattcctgtc ccttacgtgg
     9541 agaagtatgg tagtggcagc ggcagcggta gtctggaaat ccgcgcagcg tttcttgaaa
     9601 aagaaaacac cgcactgcgt acccgcgcgg ccgaactccg taaacgtgtg ggccggtgcc
     9661 gcaacattgt atccaaatac gaaacccgat acggcccgct tggttccggt tctggctctg
     9721 gttccatgga ctacaaggac catgacggtg attacaagga tcatgacatc gactacaagg
     9781 atgacgatga caag
//
