LOCUS       sequence-155896-        9713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains pBAD-CusR (response regulator) and 
            pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH015
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      101..172
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      188..215
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             716..1426
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1427..1492
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1512..1541
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1557..1574
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1630..1647)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1800..1846
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1890..1910)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1890..1908)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1929..1945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1929..1945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1942..1964)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1953..1969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1977..1983,1984..2001,2002..2007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2022..2043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(2448..3242)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2559..2578)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3169..3188
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      4722..5491
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             5492..6154
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     6846..6868
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6861..6877
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6887..6906
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6887..6905
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      6946..7032
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7127..7213
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7308..7394
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7489..7575
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7728..8606)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        8633..8917
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     8840..8859
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cggccgaact ccgtaaacgt gtgggccggt gccgcaacat tgtatccaaa tacgaaaccc
       61 gatacggccc gctttaagga tcctgactcg agccaggcat caaataaaac gaaaggctca
      121 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      181 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat accgcggcct aggattggca
      241 aaatgacaat tttgtcattt ttctgtcacc ggaaaatcag agcctggcga gtaaagttgg
      301 cggcataaaa tcaccagaaa ttatgagctc atacattcgt aagaaacggg cgcaggagga
      361 ttagatggca gagtatgtgc gggccctgtt tgactttaat gggaatgatg aagaagacct
      421 tccctttaag aaaggagaca tcctgagaat ccgggataag cctgaagagc agtggtggaa
      481 tgcagaggac agcgaaggaa agagggggat gattcctgtc ccttacgtgg agaagtatgg
      541 tagtggcagc ggcagcggta gtctggaaat ccgcgcagcg tttcttgaaa aagaaaacac
      601 cgcactgcgt acccgcgcgg ccgaactccg taaacgtgtg ggccggtgcc gcaacattgt
      661 atccaaatac gaaacccgat acggcccgct tggtagtggc agcggcagcg gtagtcgtaa
      721 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
      781 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
      841 gctgaagttc atctgtacta ctggtaaact gccggttcct tggccgactc tggtaacgac
      901 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
      961 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
     1021 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
     1081 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
     1141 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
     1201 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
     1261 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
     1321 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
     1381 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaagact acaaggacca
     1441 tgacggtgat tacaaggatc atgacatcga ctacaaggat gacgatgaca agtagtgaga
     1501 agcttgggcc cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc
     1561 atcatcatca tcattgagtt taaacggtct ccagcttggc tgttttggcg gatgagagaa
     1621 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
     1681 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
     1741 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
     1801 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1861 tgaactaatt tctagaccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     1921 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1981 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2041 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2101 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcat gcataaaaac
     2161 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     2221 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catgggggtg
     2281 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     2341 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     2401 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     2461 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     2521 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     2581 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     2641 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     2701 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     2761 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     2821 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     2881 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     2941 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     3001 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
     3061 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     3121 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     3181 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     3241 atgcgaaacg atcctcatcc tgtctcttga tcagattttg atcccctgcg ccatcagatc
     3301 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
     3361 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
     3421 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
     3481 atagcccagt agctgacatt catcccaggt ggcacttttc ggggaaatgt gcgcgcccgc
     3541 gttcctgctg gcgctgggcc tgtttctggc gctggacttc ccgctgttcc gtcagcagct
     3601 tttcgcccac ggccttgatg atcgcggcgg ccttggcctg catatcccga ttcaacggcc
     3661 ccagggcgtc cagaacgggc ttcaggcgct cccgaaggtc tcgggccgtc tcttgggctt
     3721 gatcggcctt cttgcgcatc tcacgcgctc ctgcggcggc ctgtagggca ggctcatacc
     3781 cctgccgaac cgcttttgtc agccggtcgg ccacggcttc cggcgtctca acgcgctttg
     3841 agattcccag cttttcggcc aatccctgcg gtgcataggc gcgtggctcg accgcttgcg
     3901 ggctgatggt gacgtggccc actggtggcc gctccagggc ctcgtagaac gcctgaatgc
     3961 gcgtgtgacg tgccttgctg ccctcgatgc cccgttgcag ccctagatcg gccacagcgg
     4021 ccgcaaacgt ggtctggtcg cgggtcatct gcgctttgtt gccgatgaac tccttggccg
     4081 acagcctgcc gtcctgcgtc agcggcacca cgaacgcggt catgtgcggg ctggtttcgt
     4141 cacggtggat gctggccgtc acgatgcgat ccgccccgta cttgtccgcc agccacttgt
     4201 gcgccttctc gaagaacgcc gcctgctgtt cttggctggc cgacttccac cattccgggc
     4261 tggccgtcat gacgtactcg accgccaaca cagcgtcctt gcgccgcttc tctggcagca
     4321 actcgcgcag tcggcccatc gcttcatcgg tgctgctggc cgcccagtgc tcgttctctg
     4381 gcgtcctgct ggcgtcagcg ttgggcgtct cgcgctcgcg gtaggcgtgc ttgagactgg
     4441 ccgccacgtt gcccattttc gccagcttct tgcatcgcat gatcgcgtat gccgccatgc
     4501 ctgcccctcc cttttggtgt ccaaccggct cgacgggggc agcgcaaggc ggtgcctccg
     4561 gcgggccact caatgcttga gtatactcac tagactttgc ttcgcaaagt cgtgaccgcc
     4621 tacggcggct gcggcgccct acgggcttgc tctccgggct tcgccctgcg cggtcgctgc
     4681 gctcccttgc cagcccgtgg atatgtggac gatggccgcg agcggccacc ggctggctcg
     4741 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
     4801 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
     4861 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
     4921 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
     4981 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
     5041 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
     5101 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
     5161 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
     5221 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
     5281 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
     5341 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
     5401 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
     5461 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
     5521 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
     5581 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     5641 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     5701 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     5761 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     5821 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     5881 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     5941 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     6001 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     6061 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     6121 gccgccgaca cgggtcacgc tgccgcgccg gtagcacttg ggttgcgcag caacccgtaa
     6181 gtgcgctgtt ccagactatc ggctgtagcc gcctcgccgc cctatacctt gtctgcctcc
     6241 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
     6301 cctcgctaac ggattcaccg tttttatcag gctctgggag gcagaataaa tgatcatatc
     6361 gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat ttgagaagca
     6421 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
     6481 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
     6541 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac
     6601 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtcggcctat tggttaaaaa
     6661 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6721 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6781 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     6841 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     6901 atagggcgaa ttggagctgg ggccgcaatt cccaattcca ggcatcaaat aaaacgaaag
     6961 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     7021 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     7081 cgggcaggac gcccgccata aactgccagg aattaattcc aggcatcaaa taaaacgaaa
     7141 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     7201 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     7261 gcgggcagga cgcccgccat aaactgccag gaattaattc caggcatcaa ataaaacgaa
     7321 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     7381 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     7441 ggcgggcagg acgcccgcca taaactgcca ggaattaatt ccaggcatca aataaaacga
     7501 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     7561 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     7621 tggcgggcag gacgcccgcc ataaactgcc aggaattggg gatcggaagc tgaattcact
     7681 agtctatgct actccatcga gccgtcaatt gtctgattcg ttaccaatta tgacaacttg
     7741 acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg
     7801 gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg
     7861 acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg
     7921 tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc
     7981 gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg
     8041 tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac
     8101 tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc
     8161 tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa
     8221 tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag
     8281 ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg
     8341 cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca
     8401 cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg
     8461 agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg
     8521 ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc
     8581 aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc
     8641 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta
     8701 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg
     8761 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg
     8821 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcttacc
     8881 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgctagcggt acctcacgcg
     8941 atacagatct atgaaactgt tgattgtcga agatgaaaag aaaaccggag aatacttgac
     9001 caaagggtta accgaagccg gttttgtggt cgatttggcc gacaacgggc tgaatggcta
     9061 ccatctggcg atgaccggtg attatgatct gataatcctc gatattatgc tgccggacgt
     9121 gaacggctgg gatatcgtgc gcatgttacg ctccgccaat aaagggatgc cgattctgtt
     9181 gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg ctggagttgg gggcagatga
     9241 ctacctggtg aagccattcg cttttgctga actgctggcg cgggtgcgca cattactgcg
     9301 gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt gccgatttga tggtcgatct
     9361 cgtcagccgc aaagtcaccc gcagcggcac gcgcatcact ttgaccagta aagagtttac
     9421 tctgctggag ttcttccttc gccatcaggg cgaagtgctg ccccgctcgc ttatcgcctc
     9481 gcaggtatgg gacatgaatt ttgacagcga taccaatgct attgatgtgg cggtgaagcg
     9541 gctgcgcggc aaaatcgaca acgactttga gccgaagcta attcagaccg tgcgcggcgt
     9601 gggttacatg cttgaggtgc cggatggtca gggatctggg tccggcagcg gtggatctct
     9661 ggaaatccgc gcagcgtttc ttgaaaaaga aaacaccgca ctgcgtaccc gcg
//
