LOCUS       sequence-155898-        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains pBAD-CusR (response regulator) and 
            pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH015
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             349..1059
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1060..1125
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gcagagtatg tgcgggccct gtttgacttt aatgggaatg atgaagaaga ccttcccttt
       61 aagaaaggag acatcctgag aatccgggat aagcctgaag agcagtggtg gaatgcagag
      121 gacagcgaag gaaagagggg gatgattcct gtcccttacg tggagaagta tggtagtggc
      181 agcggcagcg gtagtctgga aatccgcgca gcgtttcttg aaaaagaaaa caccgcactg
      241 cgtacccgcg cggccgaact ccgtaaacgt gtgggccggt gccgcaacat tgtatccaaa
      301 tacgaaaccc gatacggccc gcttggtagt ggcagcggca gcggtagtcg taaaggcgaa
      361 gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat
      421 aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag
      481 ttcatctgta ctactggtaa actgccggtt ccttggccga ctctggtaac gacgctgact
      541 tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag
      601 tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg
      661 tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg
      721 aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga atacaatttt
      781 aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt
      841 aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac
      901 actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc
      961 gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc
     1021 gcagcgggca tcacgcatgg tatggatgaa ctgtacaaag actacaagga ccatgacggt
     1081 gattacaagg atcatgacat cgactacaag gatgacgatg acaag
//
