LOCUS       sequence-155900-        9446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR 
            (response regulator) and pCusR-YFP-AAV..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH009
FEATURES             Location/Qualifiers
     source          1..9446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        241..525
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     448..467
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      1422..1493
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1509..1536
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             1692..1757
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2566..2595
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2611..2628
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2684..2701)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2854..2900
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2944..2964)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2944..2962)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2983..2999)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2983..2999)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2996..3018)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3007..3023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3031..3037,3038..3055,3056..3061))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3076..3097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(3502..4296)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3613..3632)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4223..4242
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      5776..6545
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             6546..7208
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     7900..7922
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7915..7931
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7941..7960
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7941..7959
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      8000..8086
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8181..8267
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8362..8448
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8543..8629
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(8782..9446,1..214))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc
       61 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
      121 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
      181 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
      241 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
      301 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      361 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      421 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      481 atcttacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ctagcggtac
      541 ctcacgcgat acagatctat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa
      601 tacttgacca aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg
      661 aatggctacc atctggcgat gaccggtgat tatgatctga taatcctcga tattatgctg
      721 ccggacgtga acggctggga tatcgtgcgc atgttacgct ccgccaataa agggatgccg
      781 attctgttgc ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg
      841 gcagatgact acctggtgaa gccattcgct tttgctgaac tgctggcgcg ggtgcgcaca
      901 ttactgcggc gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatttgatg
      961 gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc gcatcacttt gaccagtaaa
     1021 gagtttactc tgctggagtt cttccttcgc catcagggcg aagtgctgcc ccgctcgctt
     1081 atcgcctcgc aggtatggga catgaatttt gacagcgata ccaatgctat tgatgtggcg
     1141 gtgaagcggc tgcgcggcaa aatcgacaac gactttgagc cgaagctaat tcagaccgtg
     1201 cgcggcgtgg gttacatgct tgaggtgccg gatggtcagg gatctgggtc cggcagcggt
     1261 ggatctctgg aaatccgcgc agcgtttctt gaaaaagaaa acaccgcact gcgtacccgc
     1321 gcggccgaac tccgtaaacg tgtgggccgg tgccgcaaca ttgtatccaa atacgaaacc
     1381 cgatacggcc cgctttaagg atcctgactc gagccaggca tcaaataaaa cgaaaggctc
     1441 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1501 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taccgcggcc taggattggc
     1561 aaaatgacaa ttttgtcatt tttctgtcac cggaaaatca gagcctggcg agtaaagttg
     1621 gcggcataaa atcaccagaa attatgagct catacattcg taagaaacgg gcgcaggagg
     1681 attagatgat ggactacaag gaccatgacg gtgattacaa ggatcatgac atcgactaca
     1741 aggatgacga tgacaaggca actagcggca tggttagtaa aggagaagaa cttttcactg
     1801 gagttgtccc aattttagtt gaactagatg gcgacgtgaa cggtcataag ttcagtgtct
     1861 ccggcgaagg tgagggtgat gcaacgtacg gtaagttaac tttgaagtta atatgtacaa
     1921 ccggcaagct gcctgttccc tggcctaccc tggtgacaac gttaggttat gggttgatgt
     1981 gctttgctag atacccagat cacatgaaaa ggcatgactt ctttaaatct gcaatgccag
     2041 aaggttacgt ccaagaacgt actattttct ttaaagatga cggtaattat aaaactaggg
     2101 ctgaagttaa attcgaaggt gacacacttg taaatcgaat agagttaaag gggattgatt
     2161 tcaaagagga tggtaatatt ctaggccata aacttgaata taactataat tcacacaacg
     2221 tttacattac cgccgacaag cagaagaatg gaatcaaagc caattttaag attagacaca
     2281 atattgagga tggtggagta cagcttgctg atcattacca acaaaatacc ccgatcggtg
     2341 atggaccagt tttgctaccc gataaccatt atctgtccta tcaaagcaaa ttgtcaaaag
     2401 atcctaacga aaaaagagac cacatggtac tcttggaatt tgtaacagct gctgggatta
     2461 cacatggcat ggatgaacta tacaaaggtt ctggtaccgc agcagcaaac gacgaaaact
     2521 acgctgcagc agtttaataa tacaaatagt gagaagcttg ggcccgaaca aaaactcatc
     2581 tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg agtttaaacg
     2641 gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat
     2701 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     2761 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     2821 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     2881 gactgggcct ttcgttttat ctgttgtttg tcggtgaact aatttctaga ccagcttttg
     2941 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     3001 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3061 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3121 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3181 aggcggtttg cgtattgggc gcatgcataa aaactgttgt aattcattaa gcattctgcc
     3241 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     3301 gtcgccttgc gtataatatt tgcccatggg ggtgggcgaa gaactccagc atgagatccc
     3361 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     3421 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     3481 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     3541 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     3601 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     3661 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     3721 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     3781 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     3841 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     3901 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     3961 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     4021 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     4081 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     4141 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     4201 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     4261 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     4321 ttgatcagat tttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     4381 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct
     4441 tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg
     4501 ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccc
     4561 aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg ggcctgtttc
     4621 tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt gatgatcgcg
     4681 gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac gggcttcagg
     4741 cgctcccgaa ggtctcgggc cgtctcttgg gcttgatcgg ccttcttgcg catctcacgc
     4801 gctcctgcgg cggcctgtag ggcaggctca tacccctgcc gaaccgcttt tgtcagccgg
     4861 tcggccacgg cttccggcgt ctcaacgcgc tttgagattc ccagcttttc ggccaatccc
     4921 tgcggtgcat aggcgcgtgg ctcgaccgct tgcgggctga tggtgacgtg gcccactggt
     4981 ggccgctcca gggcctcgta gaacgcctga atgcgcgtgt gacgtgcctt gctgccctcg
     5041 atgccccgtt gcagccctag atcggccaca gcggccgcaa acgtggtctg gtcgcgggtc
     5101 atctgcgctt tgttgccgat gaactccttg gccgacagcc tgccgtcctg cgtcagcggc
     5161 accacgaacg cggtcatgtg cgggctggtt tcgtcacggt ggatgctggc cgtcacgatg
     5221 cgatccgccc cgtacttgtc cgccagccac ttgtgcgcct tctcgaagaa cgccgcctgc
     5281 tgttcttggc tggccgactt ccaccattcc gggctggccg tcatgacgta ctcgaccgcc
     5341 aacacagcgt ccttgcgccg cttctctggc agcaactcgc gcagtcggcc catcgcttca
     5401 tcggtgctgc tggccgccca gtgctcgttc tctggcgtcc tgctggcgtc agcgttgggc
     5461 gtctcgcgct cgcggtaggc gtgcttgaga ctggccgcca cgttgcccat tttcgccagc
     5521 ttcttgcatc gcatgatcgc gtatgccgcc atgcctgccc ctcccttttg gtgtccaacc
     5581 ggctcgacgg gggcagcgca aggcggtgcc tccggcgggc cactcaatgc ttgagtatac
     5641 tcactagact ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg ccctacgggc
     5701 ttgctctccg ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc gtggatatgt
     5761 ggacgatggc cgcgagcggc caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg
     5821 ctggacaagc tgatggacag gctgcgcctg cccacgagct tgaccacagg gattgcccac
     5881 cggctaccca gccttcgacc acatacccac cggctccaac tgcgcggcct gcggccttgc
     5941 cccatcaatt tttttaattt tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc
     6001 gcttgccggt tggacaccaa gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg
     6061 gcttggcctt gacgcgcctg gaacgaccca agcctatgcg agtgggggca gtcgaaggcg
     6121 aagcccgccc gcctgccccc cgagcctcac ggcggcgagt gcgggggttc caagggggca
     6181 gcgccacctt gggcaaggcc gaaggccgcg cagtcgatca acaagccccg gaggggccac
     6241 tttttgccgg agggggagcc gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct
     6301 ttgggcacca aagaactaga tatagggcga aatgcgaaag acttaaaaat caacaactta
     6361 aaaaaggggg gtacgcaaca gctcattgcg gcaccccccg caatagctca ttgcgtaggt
     6421 taaagaaaat ctgtaattga ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc
     6481 gaaaagttgc agctgattgc gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga
     6541 taagcatggc cacgcagtcc agagaaatcg gcattcaagc caagaacaag cccggtcact
     6601 gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca
     6661 cggcggcaat gctgctgcat cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg
     6721 tcagccagaa gacactttcc aagctcatcg gacgttcttt gcggacggtc caatacgcag
     6781 tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg
     6841 tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc
     6901 tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga ccaggacgaa tcgctgttgg
     6961 ggcatggcga cctgcgccgc atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg
     7021 gccccggcga ggagccgccc agccagcccg gcattccggg catggaacca gacctgccag
     7081 ccttgaccga aacggaggaa tgggaacggc gcgggcagca gcgcctgccg atgcccgatg
     7141 agccgtgttt tctggacgat ggcgagccgt tggagccgcc gacacgggtc acgctgccgc
     7201 gccggtagca cttgggttgc gcagcaaccc gtaagtgcgc tgttccagac tatcggctgt
     7261 agccgcctcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc
     7321 cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accgttttta
     7381 tcaggctctg ggaggcagaa taaatgatca tatcgtcaat tattacctcc acggggagag
     7441 cctgagcaaa ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca
     7501 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
     7561 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca
     7621 ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
     7681 tgccactcat cgcagtcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7741 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
     7801 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     7861 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     7921 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag ctggggccgc
     7981 aattcccaat tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     8041 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     8101 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     8161 caggaattaa ttccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     8221 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     8281 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     8341 ccaggaatta attccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     8401 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     8461 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     8521 gccaggaatt aattccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     8581 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
     8641 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
     8701 tgccaggaat tggggatcgg aagctgaatt cactagtcta tgctactcca tcgagccgtc
     8761 aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc
     8821 acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa
     8881 atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt
     8941 gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat
     9001 ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc
     9061 gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc
     9121 gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag
     9181 taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc
     9241 ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg
     9301 aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg
     9361 cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg
     9421 atgaatctct cctggcggga acagca
//
