LOCUS       sequence-155902-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR 
            (response regulator) and pCusR-YFP-AAV..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH009
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aggaccatga cggtgattac aaggatcatg acatcgacta caaggatgac
       61 gatgacaagg caactagcgg catggttagt aaaggagaag aacttttcac tggagttgtc
      121 ccaattttag ttgaactaga tggcgacgtg aacggtcata agttcagtgt ctccggcgaa
      181 ggtgagggtg atgcaacgta cggtaagtta actttgaagt taatatgtac aaccggcaag
      241 ctgcctgttc cctggcctac cctggtgaca acgttaggtt atgggttgat gtgctttgct
      301 agatacccag atcacatgaa aaggcatgac ttctttaaat ctgcaatgcc agaaggttac
      361 gtccaagaac gtactatttt ctttaaagat gacggtaatt ataaaactag ggctgaagtt
      421 aaattcgaag gtgacacact tgtaaatcga atagagttaa aggggattga tttcaaagag
      481 gatggtaata ttctaggcca taaacttgaa tataactata attcacacaa cgtttacatt
      541 accgccgaca agcagaagaa tggaatcaaa gccaatttta agattagaca caatattgag
      601 gatggtggag tacagcttgc tgatcattac caacaaaata ccccgatcgg tgatggacca
      661 gttttgctac ccgataacca ttatctgtcc tatcaaagca aattgtcaaa agatcctaac
      721 gaaaaaagag accacatggt actcttggaa tttgtaacag ctgctgggat tacacatggc
      781 atggatgaac tatacaaagg ttctggtacc gcagcagcaa acgacgaaaa ctacgctgca
      841 gcagtttaat aa
//
