LOCUS       sequence-155911-        8765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent 
            protein. Used as a control for autofluorescence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH004
FEATURES             Location/Qualifiers
     source          1..8765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      706..1475
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1476..2138
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2830..2852
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2845..2861
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2871..2890
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2871..2889
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2930..3016
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3111..3197
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3292..3378
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3473..3559
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3712..4590)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4617..4901
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4824..4843
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      5798..5869
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5885..5912
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             6261..6290
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6306..6323
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6379..6396)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6549..6595
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(6639..6659)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6639..6657)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6678..6694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6678..6694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6691..6713)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6702..6718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6726..6732,6733..6750,6751..6756))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6771..6792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(7197..7991)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7308..7327)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7918..7937
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcggccgcaa acgtggtctg gtcgcgggtc atctgcgctt tgttgccgat gaactccttg
       61 gccgacagcc tgccgtcctg cgtcagcggc accacgaacg cggtcatgtg cgggctggtt
      121 tcgtcacggt ggatgctggc cgtcacgatg cgatccgccc cgtacttgtc cgccagccac
      181 ttgtgcgcct tctcgaagaa cgccgcctgc tgttcttggc tggccgactt ccaccattcc
      241 gggctggccg tcatgacgta ctcgaccgcc aacacagcgt ccttgcgccg cttctctggc
      301 agcaactcgc gcagtcggcc catcgcttca tcggtgctgc tggccgccca gtgctcgttc
      361 tctggcgtcc tgctggcgtc agcgttgggc gtctcgcgct cgcggtaggc gtgcttgaga
      421 ctggccgcca cgttgcccat tttcgccagc ttcttgcatc gcatgatcgc gtatgccgcc
      481 atgcctgccc ctcccttttg gtgtccaacc ggctcgacgg gggcagcgca aggcggtgcc
      541 tccggcgggc cactcaatgc ttgagtatac tcactagact ttgcttcgca aagtcgtgac
      601 cgcctacggc ggctgcggcg ccctacgggc ttgctctccg ggcttcgccc tgcgcggtcg
      661 ctgcgctccc ttgccagccc gtggatatgt ggacgatggc cgcgagcggc caccggctgg
      721 ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc tgatggacag gctgcgcctg
      781 cccacgagct tgaccacagg gattgcccac cggctaccca gccttcgacc acatacccac
      841 cggctccaac tgcgcggcct gcggccttgc cccatcaatt tttttaattt tctctgggga
      901 aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa gtggaaggcg
      961 ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg gaacgaccca
     1021 agcctatgcg agtgggggca gtcgaaggcg aagcccgccc gcctgccccc cgagcctcac
     1081 ggcggcgagt gcgggggttc caagggggca gcgccacctt gggcaaggcc gaaggccgcg
     1141 cagtcgatca acaagccccg gaggggccac tttttgccgg agggggagcc gcgccgaagg
     1201 cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca aagaactaga tatagggcga
     1261 aatgcgaaag acttaaaaat caacaactta aaaaaggggg gtacgcaaca gctcattgcg
     1321 gcaccccccg caatagctca ttgcgtaggt taaagaaaat ctgtaattga ctgccacttt
     1381 tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc agctgattgc gcatggtgcc
     1441 gcaaccgtgc ggcaccctac cgcatggaga taagcatggc cacgcagtcc agagaaatcg
     1501 gcattcaagc caagaacaag cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg
     1561 cgtgggccgg gcttattgcg aggaaaccca cggcggcaat gctgctgcat cacctcgtgg
     1621 cgcagatggg ccaccagaac gccgtggtgg tcagccagaa gacactttcc aagctcatcg
     1681 gacgttcttt gcggacggtc caatacgcag tcaaggactt ggtggccgag cgctggatct
     1741 ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta cgtggtcaat gaccgcgtgg
     1801 cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg
     1861 atcacgacga ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc
     1921 tgtatccggg cgagcagcaa ctaccgaccg gccccggcga ggagccgccc agccagcccg
     1981 gcattccggg catggaacca gacctgccag ccttgaccga aacggaggaa tgggaacggc
     2041 gcgggcagca gcgcctgccg atgcccgatg agccgtgttt tctggacgat ggcgagccgt
     2101 tggagccgcc gacacgggtc acgctgccgc gccggtagca cttgggttgc gcagcaaccc
     2161 gtaagtgcgc tgttccagac tatcggctgt agccgcctcg ccgccctata ccttgtctgc
     2221 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     2281 ggcacctcgc taacggattc accgttttta tcaggctctg ggaggcagaa taaatgatca
     2341 tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga
     2401 agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag
     2461 cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct
     2521 gccattcatc cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa
     2581 taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtcggc ctattggtta
     2641 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     2701 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     2761 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     2821 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     2881 cactataggg cgaattggag ctggggccgc aattcccaat tccaggcatc aaataaaacg
     2941 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     3001 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     3061 gtggcgggca ggacgcccgc cataaactgc caggaattaa ttccaggcat caaataaaac
     3121 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     3181 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     3241 ggtggcgggc aggacgcccg ccataaactg ccaggaatta attccaggca tcaaataaaa
     3301 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     3361 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     3421 gggtggcggg caggacgccc gccataaact gccaggaatt aattccaggc atcaaataaa
     3481 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3541 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     3601 agggtggcgg gcaggacgcc cgccataaac tgccaggaat tggggatcgg aagctgaatt
     3661 cactagtcta tgctactcca tcgagccgtc aattgtctga ttcgttacca attatgacaa
     3721 cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc
     3781 cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg
     3841 accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg
     3901 ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag
     3961 acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc
     4021 caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag
     4081 cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag
     4141 cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct
     4201 gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg
     4261 ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca
     4321 ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat
     4381 atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat
     4441 ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata
     4501 accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg
     4561 cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga
     4621 aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc
     4681 gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc
     4741 catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta
     4801 tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat tagcggatct
     4861 tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgctag cggtacctca
     4921 cgcgatacag atctatgaaa ctgttgattg tcgaagatga aaagaaaacc ggagaatact
     4981 tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac gggctgaatg
     5041 gctaccatct ggcgatgacc ggtgattatg atctgataat cctcgatatt atgctgccgg
     5101 acgtgaacgg ctgggatatc gtgcgcatgt tacgctccgc caataaaggg atgccgattc
     5161 tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag ttgggggcag
     5221 atgactacct ggtgaagcca ttcgcttttg ctgaactgct ggcgcgggtg cgcacattac
     5281 tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat ttgatggtcg
     5341 atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactttgacc agtaaagagt
     5401 ttactctgct ggagttcttc cttcgccatc agggcgaagt gctgccccgc tcgcttatcg
     5461 cctcgcaggt atgggacatg aattttgaca gcgataccaa tgctattgat gtggcggtga
     5521 agcggctgcg cggcaaaatc gacaacgact ttgagccgaa gctaattcag accgtgcgcg
     5581 gcgtgggtta catgcttgag gtgccggatg gtcagggatc tgggtccggc agcggtggat
     5641 ctctggaaat ccgcgcagcg tttcttgaaa aagaaaacac cgcactgcgt acccgcgcgg
     5701 ccgaactccg taaacgtgtg ggccggtgcc gcaacattgt atccaaatac gaaacccgat
     5761 acggcccgct ttaaggatcc tgactcgagc caggcatcaa ataaaacgaa aggctcagtc
     5821 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     5881 cactggctca ccttcgggtg ggcctttctg cgtttatacc gcggcctagg attggcaaaa
     5941 tgacaatttt gtcatttttc tgtcaccgga aaatcagagc ctggcgagta aagttggcgg
     6001 cataaaatca ccagaaatta tgagctcata cattcgtaag aaacgggcgc aggaggatta
     6061 gatggcagag tatgtgcggg ccctgtttga ctttaatggg aatgatgaag aagaccttcc
     6121 ctttaagaaa ggagacatcc tgagaatccg ggataagcct gaagagcagt ggtggaatgc
     6181 agaggacagc gaaggaaaga gggggatgat tcctgtccct tacgtggaga agtattacaa
     6241 atagtgagaa gcttgggccc gaacaaaaac tcatctcaga agaggatctg aatagcgccg
     6301 tcgaccatca tcatcatcat cattgagttt aaacggtctc cagcttggct gttttggcgg
     6361 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     6421 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     6481 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     6541 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     6601 gtttgtcggt gaactaattt ctagaccagc ttttgttccc tttagtgagg gttaattgcg
     6661 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6721 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6781 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6841 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgcatg
     6901 cataaaaact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     6961 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     7021 atgggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     7081 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     7141 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     7201 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     7261 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     7321 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7381 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     7441 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7501 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7561 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     7621 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     7681 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     7741 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     7801 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7861 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7921 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7981 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagattttga tcccctgcgc
     8041 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     8101 ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct
     8161 agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc
     8221 cttgtccaga tagcccagta gctgacattc atcccaggtg gcacttttcg gggaaatgtg
     8281 cgcgcccgcg ttcctgctgg cgctgggcct gtttctggcg ctggacttcc cgctgttccg
     8341 tcagcagctt ttcgcccacg gccttgatga tcgcggcggc cttggcctgc atatcccgat
     8401 tcaacggccc cagggcgtcc agaacgggct tcaggcgctc ccgaaggtct cgggccgtct
     8461 cttgggcttg atcggccttc ttgcgcatct cacgcgctcc tgcggcggcc tgtagggcag
     8521 gctcataccc ctgccgaacc gcttttgtca gccggtcggc cacggcttcc ggcgtctcaa
     8581 cgcgctttga gattcccagc ttttcggcca atccctgcgg tgcataggcg cgtggctcga
     8641 ccgcttgcgg gctgatggtg acgtggccca ctggtggccg ctccagggcc tcgtagaacg
     8701 cctgaatgcg cgtgtgacgt gccttgctgc cctcgatgcc ccgttgcagc cctagatcgg
     8761 ccaca
//
