LOCUS       sequence-155916-        5161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Creasy CL, Ambrose DM, Chernoff J
  TITLE     The Ste20-like protein kinase, Mst1, dimerizes and contains an 
            inhibitory domain.
  JOURNAL   J Biol Chem. 1996 Aug 30. 271(35):21049-53.
  PUBMED    8702870
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1996 Aug 30. 271(35):21049-53."
COMMENT     Sequence Label: pJ3M-Mst1 K59R
FEATURES             Location/Qualifiers
     source          1..5161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..145
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(71..90)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     231..253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(396..416)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        401..730
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      581..716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             750..779
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          2399..2464
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2594..2614
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3039..3173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3076..3095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3130..3149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3204..3308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3309..3377,3378..4169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3527..3546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4340..4928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4829..4848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5082..5099
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      121 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctag aaactgctga
      301 gggctggacc gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt
      361 accacagata tcctgtttgg cccatattca gctgtggaat gtgtgtcagt tagggtgtgg
      421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      481 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      541 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      721 cttttgcaaa agcttcgatc gtcgacatgg aacagaaact catctctgaa gaggatctgt
      781 ctagaggatc cgccatggag acggtacagc tgaggaaccc gccgcgccgg cagctgaaaa
      841 agttggatga agatagttta accaaacaac cagaagaagt atttgatgtc ttagagaaac
      901 ttggagaagg gtcctatggc agcgtataca aagctattca taaagagacc ggccagattg
      961 ttgctattag gcaagttcct gtggaatcag acctccagga gataatcaaa gaaatctcta
     1021 taatgcagca atgtgacagc cctcatgtag tcaaatatta tggcagttat tttaagaaca
     1081 cagacttatg gatcgttatg gagtactgtg gggctggttc tgtatctgat atcattcgat
     1141 tacgaaataa aacgttaaca gaagatgaaa tagctacaat attacaatca actcttaagg
     1201 gacttgaata ccttcatttt atgagaaaaa tacaccgaga tatcaaggca ggaaatattt
     1261 tgctaaatac agaaggacat gcaaaacttg cagattttgg ggtagcaggt caacttacag
     1321 ataccatggc caagcggaat acagtgatag gaacaccatt ttggatggct ccagaagtga
     1381 ttcaggaaat tggatacaac tgtgtagcag acatctggtc cctgggaata actgccatag
     1441 aaatggctga aggaaagccc ccttatgctg atatccatcc aatgagggca atcttcatga
     1501 ttcctacaaa tcctcctccc acattccgaa aaccagagct atggtcagat aactttacag
     1561 attttgtgaa acagtgtctt gtaaagagcc ctgagcagag ggccacagcc actcagctcc
     1621 tgcagcaccc atttgtcagg agtgccaaag gagtgtcaat actgcgagac ttaattaatg
     1681 aagccatgga tgtgaaactg aaacgccagg aatcccagca gcgggaagtg gaccaggacg
     1741 atgaagaaaa ctcagaagag gatgaaatgg attctggcac gatggttcga gcagtgggtg
     1801 atgagatggg cactgtccga gtagccagca ccatgactga tggagccaat actatgattg
     1861 agcacgatga cacgttgcca tcacaactgg gcaccatggt gatcaatgca gaggatgagg
     1921 aagaggaagg aactatgaaa agaagggatg agaccatgca gcctgcgaaa ccatcctttc
     1981 ttgaatattt tgaacaaaaa gaaaaggaaa accagatcaa cagctttggc aagagtgtac
     2041 ctggtccact gaaaaattct tcagattgga aaataccaca ggatggagac tacgagtttc
     2101 ttaagagttg gacagtggag gaccttcaga agaggctctt ggccctggac cccatgatgg
     2161 agcaggagat tgaagagatc cggcagaagt accagtccaa gcggcagccc atcctggatg
     2221 ccatagaggc taagaagaga cggcaacaaa acttctgagc aaggccaggc tgtgagggcc
     2281 ccagctccac ccaggctttg ggtgaattca tcgatggtac cagatctttg tgaaggaacc
     2341 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     2401 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     2461 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     2521 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     2581 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     2641 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     2701 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     2761 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     2821 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     2881 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     2941 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     3001 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     3061 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3121 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctctgcag
     3181 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3241 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3301 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3361 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3421 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3481 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3541 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3601 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3661 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3721 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3781 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3841 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3901 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3961 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4021 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4081 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4141 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4201 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4261 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4321 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     4501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4981 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5041 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5101 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5161 g
//
