LOCUS       sequence-155926-        1527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Hsiao V, Hori Y, Rothemund PW, Murray RM
  TITLE     A population-based temporal logic gate for timing and recording 
            chemical events.
  JOURNAL   Mol Syst Biol. 2016 May 17;12(5):869. doi: 10.15252/msb.20156663.
  PUBMED    27193783
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2016-05-17"; volume: "12(5):869. doi"; pages: " 
            10.15252/msb.20156663"
COMMENT     Sequence Label: pVHed05
FEATURES             Location/Qualifiers
     source          1..1527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1492..1524
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag
                     /note="wild-type LAA variant"
                     /translation="AAKDENYALAA"
ORIGIN
        1 atgaaacatc atcaccatca ccaccaggcc ggcactaaga aagtagcaat ctatacacga
       61 gtatccacta ctaaccaagc agaggaaggc ttctcaattg atgagcaaat tgaccgttta
      121 acaaaatatg ctgaagcaat ggggtggcaa gtatctgata cttatactga tgctggtttt
      181 tcaggggcca aacttgaacg cccagcaatg caaagattaa tcaacgatat cgagaataaa
      241 gcttttgata cagttcttgt atataagcta gaccgccttt cacgtagtgt aagagatact
      301 ctttatcttg ttaaggatgt gttcacaaaa aataaaatag actttatctc gcttaatgaa
      361 agtattgata cttcttctgc tatgggtagc ttgtttctca ctattctttc tgcaattaat
      421 gagtttgaaa gagagaatat aaaagaacgc atgactatgg gtaaactagg gcgagcgaaa
      481 tctggtaagt ctatgatgtg gactaagaca gcttttgggt attaccacaa cagaaagaca
      541 ggtatattag aaattgttcc tttacaagct acaatagttg aacaaatatt cactgattat
      601 ttatcaggaa tatcacttac aaaattaaga gataaactca atgaatctgg acacatcggt
      661 aaagatatac cgtggtctta tcgtacccta agacaaacac ttgataatcc agtttactgt
      721 ggttatatca aatttaagga cagcctattt gaaggtatgc acaaaccaat tatcccttat
      781 gagacttatt taaaagttca aaaagagcta gaagaaagac aacagcagac ttatgaaaga
      841 aataacaacc ctagaccttt ccaagctaaa tatatgctgt cagggatggc aaggtgcggt
      901 tactgtggag cacctttaaa aattgttctt ggccacaaaa gaaaagatgg aagccgcact
      961 atgaaatatc actgtgcaaa tagatttcct cgaaaaacaa aaggaattac agtatataat
     1021 gacaataaaa agtgtgattc aggaacttat gatttaagta atttagaaaa tactgttatt
     1081 gacaacctga ttggatttca agaaaataat gactccttat tgaaaattat caatggcaac
     1141 aaccaaccta ttcttgatac ttcgtcattt aaaaagcaaa tttcacagat cgataaaaaa
     1201 atacaaaaga actctgattt gtacctaaat gattttatca ctatggatga gttgaaagat
     1261 cgtactgatt cccttcaggc tgagaaaaag ctgcttaaag ctaagattag cgaaaataaa
     1321 tttaatgact ctactgatgt ttttgagtta gttaaaactc agttgggctc aattccgatt
     1381 aatgaactat catatgataa taaaaagaaa atcgtcaaca accttgtatc aaaggttgat
     1441 gttactgctg ataatgtaga tatcatattt aaattccaac tcgctaccgg tgctgctaag
     1501 gacgaaaact acgctctggc tgcttaa
//
