LOCUS       sequence-155931-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Hsiao V, Hori Y, Rothemund PW, Murray RM
  TITLE     A population-based temporal logic gate for timing and recording 
            chemical events.
  JOURNAL   Mol Syst Biol. 2016 May 17;12(5):869. doi: 10.15252/msb.20156663.
  PUBMED    27193783
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2016-05-17"; volume: "12(5):869. doi"; pages: " 
            10.15252/msb.20156663"
COMMENT     Sequence Label: pVHed02
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGTKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
       61 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
      121 ctgacgctga agttcatctg tactactggt aaactgccgg taccttggcc gactctggta
      181 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
      241 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
      301 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac tctggtaaac
      361 cgcattgagc tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg
      421 gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcact
      481 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
      541 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
      601 agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
      661 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgatga
//
