LOCUS       sequence-155934-        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-tagged SLC30A10-D248A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Zogzas CE, Aschner M, Mukhopadhyay S
  TITLE     Structural Elements in the Transmembrane and Cytoplasmic Domains of 
            the Metal Transporter SLC30A10 Are Required for Its Manganese Efflux
            Activity.
  JOURNAL   J Biol Chem. 2016 Jul 29;291(31):15940-57. doi: 
            10.1074/jbc.M116.726935. Epub 2016 Jun 15.
  PUBMED    27307044
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.726935"; journalName: "J Biol Chem"; date: 
            "2016-07-29- 29"; volume: "291"; issue: "31"; pages: "15940-57"
COMMENT     Sequence Label: FLAG-SLC30A10-D248A
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     gap             1149..1159
                     /estimated_length=11
     gap             1162..1176
                     /estimated_length=15
     gap             1197..1219
                     /estimated_length=23
ORIGIN
        1 ctcgttnagt gaaccgtcag aattnnacca tggactacaa agaccatgac ggtgattata
       61 aannnnnnng acatcgatta caaggatgac gatgacaagc ttgcggccgc gaattcgatg
      121 ggccgctact ctggcaagac gtgccggctg ctcttcatgc tggtgctcac cgtcgccttc
      181 ttcgtggcnn annnggtctc cggctacctg ggcaactcca tcgcgctgct ctccgactcc
      241 ttcaacatgc tctccgacct gatctcgctg tgcgtgggcc tgagcgccgg ctacatcgcc
      301 cggcgcccca cccggggctt cagcgccacc tacggctacg cccgcgccga ggtggtgggc
      361 gcgctgagca acgcggtctt cctcaccgcg ctctgcttca ccatcttcgt ggaggccgtg
      421 ctgcgcctgg cccggcccga gcgcatcgat gaccccgagc tggtgctcat cgtcggcgtc
      481 ctggggctgt tggtcaacgt ggtggggctg ctcatcttcc aggactgcgc cgcctggttc
      541 gcgtgctgcc tccggggacg cagtcgccgc ctgcagcagc ggcagcagct ggcggagggc
      601 tgtgtccccg gcgctttcgg ggggcctcag ggcgcgnang anccgcggcg cgcggcggac
      661 ccgacagccc caggctcgga ctcggccgta accctccggg ggacctcngt ggaaaggaag
      721 cgggagaagg gggcgaccgt gttcgcaaac gtagcangtg attccttcaa cacccagaat
      781 gagccagaag acatgatgaa aaaagagaaa aagtctgaag ctctgaatat cagangagta
      841 cttttgcatg tgatgggagc tgccctgggg tccgtggttg tggtcatcac ggccatcata
      901 ttctatgtgc ttcccctgaa gagtgaggac ccgtgtaact ggcagtgtta cattgacccc
      961 agcctgactg tcctcatggt catcatcatt ttgtcatctg ncnncccgct tatcaaggan
     1021 accgctgccn tnngctannn nnggtcccna agnantnnan catggnnnnn ctganganta
     1081 aacnnnntgc tnnnnctnnn nnncannnnn acnnnnannn nncntntnnn nnntnntnan
     1141 nnnnnanann nnnnnnnnnc cnnnnnnnnn nnnnnncnnn nnnnnggggn nnntnannnn
     1201 nnnnnnnnnn nnnnnnnnnc nnnnnnggna nnnc
//
