LOCUS       sequence-155935-        1218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-tagged SLC30A10-E25A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Zogzas CE, Aschner M, Mukhopadhyay S
  TITLE     Structural Elements in the Transmembrane and Cytoplasmic Domains of 
            the Metal Transporter SLC30A10 Are Required for Its Manganese Efflux
            Activity.
  JOURNAL   J Biol Chem. 2016 Jul 29;291(31):15940-57. doi: 
            10.1074/jbc.M116.726935. Epub 2016 Jun 15.
  PUBMED    27307044
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.726935"; journalName: "J Biol Chem"; date: 
            "2016-07-29- 29"; volume: "291"; issue: "31"; pages: "15940-57"
COMMENT     Sequence Label: FLAG-SLC30A10-E25A
FEATURES             Location/Qualifiers
     source          1..1218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..102
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     gap             1019..1029
                     /estimated_length=11
     gap             1093..1106
                     /estimated_length=14
     gap             1108..1117
                     /estimated_length=10
     gap             1119..1139
                     /estimated_length=21
     gap             1145..1157
                     /estimated_length=13
     gap             1166..1178
                     /estimated_length=13
     gap             1181..1194
                     /estimated_length=14
ORIGIN
        1 gnnganctcg tttagtgacc gtcagaatta accntggact acaaagacca tgacggtgat
       61 tataaagatc atgacatcga ttacaaggat gacgatgaca agcttgcggc cgcgaattcg
      121 atgggccgct actctggcaa gacgtgccgg ctgctcttca tgctggtgct caccgtcgcc
      181 ttcttcgtgg cggcgctggt ctccggctac ctgggcaact ccatcgcgct gctctccgac
      241 tccttcaaca tgctctccga cctgatctcg ctgtgcgtgg gcctgagcgc cggctacatc
      301 gcccggcgcc ccacccgggg cttcagcgcc acctacggct acgcccgcgc cgaggtggtg
      361 ggcgcgctga gcaacgcggt cttcctcacc gcgctctgct tcaccatctt cgtggaggcc
      421 gtgctgcgcc tggcccggcc cgagcgcatc gatgaccccg agctggtgct catcgtcggc
      481 gtcctggggc tgttggtcaa cgtggtgggg ctgctcatct tccaggactg cgccgcctgg
      541 ttcgcgtgct gcctccgggg acgcagtcgc cgcctgcagc agcggcagca gctggcggan
      601 ggctgtgtcc ccggcgcttt cggggggcct cagggcgcgg aggacccgcg gcgcgcggcg
      661 ganccgacag ccccaggctc ggactcggcc gtaaccctcc ngggganctc ggtggaaagg
      721 aagcgggaga anggggcgac cgtgttcgca aacgtagcnn ngattccttc nacacccaga
      781 atgagccana agacatgatg aaaaaagaga aaaagtctga agctctgaat atcanagntg
      841 tacttttgca tgtgatggga gatgccctgg ggtccgtggn tgtggtcatc acggccatca
      901 tattctatgt gcttnccctg aanantgang acccgtgtaa ctggcnntgn tacattgacc
      961 ccngcctgac tgtcntcatg gncatcatca tttnncatct gnnnncccgc tnatcangnn
     1021 nnnnnnnnnc nttctgctnc nnanngnncn cnaaagnant caanatgnnn nnnctgannn
     1081 antaannnnn ntnnnnnnnn nnnnnncnnn nnnnnnnann nnnnnnnnnn nnnnnnnnng
     1141 nnaannnnnn nnnnnnncnn nnnncnnnnn nnnnnnnngg nnnnnnnnnn nnnnannnnn
     1201 nannnnntcn cnnnnnng
//
