LOCUS       sequence-155936-        1246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-tagged SLC30A10-N43H in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Zogzas CE, Aschner M, Mukhopadhyay S
  TITLE     Structural Elements in the Transmembrane and Cytoplasmic Domains of 
            the Metal Transporter SLC30A10 Are Required for Its Manganese Efflux
            Activity.
  JOURNAL   J Biol Chem. 2016 Jul 29;291(31):15940-57. doi: 
            10.1074/jbc.M116.726935. Epub 2016 Jun 15.
  PUBMED    27307044
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.726935"; journalName: "J Biol Chem"; date: 
            "2016-07-29- 29"; volume: "291"; issue: "31"; pages: "15940-57"
COMMENT     Sequence Label: FLAG-SLC30A10-N43H
FEATURES             Location/Qualifiers
     source          1..1246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     gap             1145..1154
                     /estimated_length=10
     gap             1187..1203
                     /estimated_length=17
     gap             1235..1244
                     /estimated_length=10
ORIGIN
        1 gnngagctcg tttagtgaac cgtcagaatt aaccatggac tacaaagacc atgacggtga
       61 ttataaagat catgacatcg attacaagga tgacgatgac aagcttgcgg ccgcgaattc
      121 gatgggccgc tactctggca agacgtgccg gctgctcttc atgctggtgc tcaccgtcgc
      181 cttcttcgtg gcggagctgg tctccggcta cctgggcaac tccatcgcgc tgctctccga
      241 cagcttccat atgctctccg acctgatctc gctgtgcgtg ggcctgagcg ccggctacat
      301 cgcccggcgc cccacccggg gcttcagcgc cacctacggc tacgcccgcg ccgaggtggt
      361 gggcgcgctg agcaacgcgg tcttcctcac cgcgctctgc ttcaccatct tcgtggaggc
      421 cgtgctgcgc ctggcccggc ccgagcgcat cgatgacccc gagctggtgc tcatcgtcgg
      481 cgtcctgggg ctgttggtca acgtggtggg gctgctcatc ttccaggact gcgccgcctg
      541 gttcgcgtgc tgcctccggg gacgcagtcg ccgcctgcag cagcggcagc agctggcgga
      601 gggctgtgtc cccggcgctt tcggggggcc tcagggcgcg gaggacccgc ggcgcgcggc
      661 ggacccgaca gccccaggct cggactcggc cgtaaccctc cgggggacct cggtggnnng
      721 aagcgggaga agggggcgac cgtgttcgca aacgtagcnn tgattccttc nacacccaga
      781 atgagccaga agacatgatg aaaaaagaga aaaagtctga agctctgaat atcagaggtg
      841 tacttttgca tgtgatggga gatgccctgg ggtccgtggn tgtggtcatc acggccatca
      901 tattctatgt gcttcccctg aagagtgagg acccgtgtaa ctggcagtgn tacattgacc
      961 ccagcctgac tgtcctcntg gncatcatca ttttgtcntc tgnnntcccn nttatcangg
     1021 anacnnctgc cattnngcta cngatggnnc ccnaannnng tcaanntnnn nnnnnctgat
     1081 gantnaacnn nnntnctgnn nctngnanna gccnnnnnnn nntnnannnn nnnntnnnnn
     1141 nnctnnnnnn nnnnaanatt nntnnnnncn nnnnnnnnan nnnnccnnnn nnnnnnnnnn
     1201 nnnanannnc nnnnnnnaan nnnnnnaanc ntncnnnnnn nnnngg
//
