LOCUS       sequence-155954-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 
            2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). 
            Also contains bicistronially expressed cytoplasmic red reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: 2PH-PLCd-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    44..64
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(70..765)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
ORIGIN
        1 gatctacgta atacgactca ctatagttct agaggctcga gtgcaactat gtataataaa
       61 gttgactagt catctgtgcc ccagtttgct agggaggtcg cagtatctgg ccacagccac
      121 ctcgtgctgc tcgacgtagg tctctttgtc ggcctccttg attctttcca gtcttctgtc
      181 cacatagtag acgccgggca tcttgaggtt cttagcgggt ttcttggatc tgtatgtggt
      241 cttcaagttg cagatcaggt ggcccccgcc cacgagcttc agggccatgt cggctctgcc
      301 ttccaggccg ccgtcagcgg ggtacagggt ctcggtggag gcctcccagc cgagtgtttt
      361 cttctgcatc acagggccgt tggatgggaa gttcacccct ctgatcttga cgttgtagat
      421 gaggcagccg tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta
      481 tgtggtgact ctctcccatg tgaagccctc ggggaaggac tgcttaaaga agtcggggat
      541 gccctgggtg tggttgatga aggttttgct gccgtacatg aagctggtag ccaggatgtc
      601 gaaggcgaag gggagagggc cgccctcgac cgccttgatt ctcatggtct gggtgccctc
      661 gtagggcttg ccttcgccct cggatgtgca cttgaagtgg tggttgttca cggtgccctc
      721 catgtacagc ttcatgtgca tgttctcctt aatcagctcg ctcacggtac cgcttccagg
      781 agcagga
//
