LOCUS       sequence-155955-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A118 attL-attR. GFP expression will stop if sites recombine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR280
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        187..236
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             269..280
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(317..345)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata aggctcgtat
       61 aatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt aactttttaa
      121 ttaacggccg aacttttcgg atcaagctat gaaggaagaa gaagaaacga gaaactaaat
      181 gcacagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
      241 tacaaataat tttgtttaag aattcattaa agaggagaaa ggtaccatga gtaaaggaga
      301 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      361 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      421 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      481 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      541 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      601 ctacaagaca cgtgctgaag tcaagtttga a
//
