LOCUS       sequence-155956-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  piggyBac transposon vector, Zeocin-2A-sHRPbNLG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..145)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      589..598
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(627..646)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 actgaatgac aggacccaaa atttcattat tgagttcttt cttaattccc ctaatcttgc
       61 caaagttagt agtaaccaat gggtctacat catccaacaa ttgggtgctg tccaggccca
      121 gcagggggtt ggggatgggc ttgccgcccg agcccgagcc agagttgctg ttcacgaccc
      181 tacagttcag tcttatctgt ccctgcgtac ccgtcagggg cgttatattt cccatccgat
      241 ccatagcttc cacgaaagca ttgaaaaagg tttgtgtgct attagcgaag ctgcgcacaa
      301 gaggaatagt gtcggtagca tttggggaac tgaacagctc ctggtcggac tgaatcaggc
      361 ccttctgttc ctccagattg acgtaatact tattgtcgaa gatggttggg gtgcgcaggt
      421 caaagtcaac cagtgcagac aagttaccgg tcttttgaga gagtacgtga aaagtctgta
      481 gcaaacatcc caacaagcag agagtcaatg gggcaccgga tcccctgtgg accacacatg
      541 ccatcatagc tctccaaaca taatttggcc acatgcatct gggaagtgcc atggtggcgg
      601 aattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag gagctgtagg
      661 aaaaagaaga aggcatgaac atcgcggccg cgatatctgg ccctgggtta gactcaacgt
      721 cccctgccaa cttcaacaag tcaaagttag gtccaggatt ggattcgaca tctccagcga
      781 gtttgaggag atcaaaatta ggtccaggat tgctctccac gtc
//
