LOCUS       sequence-155960-         443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_R0040. [A:36INS-pTet:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-pTet_AB
FEATURES             Location/Qualifiers
     source          1..443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    34..52
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    107..128
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      131..174
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(178..196)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        264..368
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctcagtatc tctatcactg atagggatgt caatctctat cactgatagg gaacaacagt
       61 gaggtttctt tactatgtat tctctccgct cctgagaccc actagtctct agaagcggcc
      121 gcgaattcca gaaatcatcc ttagcgaaag ctaaggattt tttttatctg aaattctgcc
      181 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      241 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      301 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      361 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      421 gccttcctgt ttttgctcac cca
//
