LOCUS       sequence-155961-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_R0040. [E:36INS-pTet:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-pTet_EB
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..90
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    170..188
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    210..230
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(236..253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(487..506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagag actagtgggt ctcagcttcg gagagaatac
      121 atagtaaaga aacctcactg ttgttcccta tcagtgatag agattgacat ccctatcagt
      181 gatagagata ctgagcacta ctagagacct actagtagcg gccgctgcag gcttcctcgc
      241 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      301 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
      421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      601 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      781 gagcgaggta tgtaggcggt gctacagagt tcttga
//
