LOCUS       sequence-155964-       12012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12012)
  AUTHORS   Wei Q, Adelstein RS
  TITLE     Conditional expression of a truncated fragment of nonmuscle myosin 
            II-A alters cell shape but not cytokinesis in HeLa cells.
  JOURNAL   Mol Biol Cell. 2000 Oct . 11(10):3617-27.
  PUBMED    11029059
REFERENCE   2  (bases 1 to 12012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2000 Oct . 11(10):3617-27."
COMMENT     Sequence Label: pTRE-GFP-NMHC II-B
FEATURES             Location/Qualifiers
     source          1..12012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..97
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    163..181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    247..265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        298..336
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     333..357
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      436..445
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(442..444,445..447,448..1158)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(442..444,445..447,448..1158)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(487..508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(748..767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1095..1116
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8640..8656)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8640..8656)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    8807..8928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8844..8863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8898..8917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8935..9390)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9072..9093)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9284..9303
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9417..9521
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9523..9880
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9523..9714
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9546..9566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9731..9866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9915..10709
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9915..10709
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9969..9988)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10579..10598
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10897..10916)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10941..10988
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11317..11905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11806..11825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatcg tcctcgagtt taccactccc tatcagtgat agagaaaagt
       61 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      121 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      181 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      241 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
      301 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      361 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      421 cgagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
      481 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      541 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      601 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      661 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      721 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      781 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      841 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      901 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      961 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1021 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     1081 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1141 atggacgagc tgtacaagta ctcagatctc gagctcaagc ttcgaattcc aatggcgcag
     1201 agaactggac tcgaggatcc agagaggtat ctctttgtgg acagggctgt catctacaac
     1261 cctgccactc aagctgattg gacagctaaa aagctagtgt ggattccatc agaacgccat
     1321 ggttttgagg cagctagtat caaagaagaa cggggagatg aagttatggt ggagttggca
     1381 gagaatggaa agaaagcaat ggtcaacaaa gatgatattc agaagatgaa cccacctaag
     1441 ttttccaagg tggaggatat ggcagaattg acatgcttga atgaagcttc cgttttacat
     1501 aatctgaagg atcgctacta ttcaggacta atctatactt attctggact cttctgtgta
     1561 gttataaacc cttacaagaa tcttccaatt tactctgaga atattattga aatgtacaga
     1621 gggaagaagc gtcatgagat gcctccacac atctatgcta tatctgaatc tgcttacaga
     1681 tgcatgcttc aagatcgtga ggaccagtca attctttgca cgggtgagtc aggtgctggg
     1741 aagacagaaa atacaaagaa agttattcag taccttgccc atgttgcttc ttcacataaa
     1801 ggaagaaagg accataatat tcctggggaa cttgaacggc agcttttgca agcaaatcca
     1861 attctggaat catttggaaa tgcgaagact gtgaaaaatg ataactcatc tcgttttggc
     1921 aaatttattc ggatcaactt tgatgtaact ggctatatcg ttggggccaa cattgaaaca
     1981 taccttctgg aaaagtctcg tgctgttcgt caagcaaaag atgaacgtac ttttcatatc
     2041 ttttaccagt tgttatctgg agcaggagaa cacctaaagt ctgatttgct tcttgaagga
     2101 tttaataact acaggtttct ctccaatggc tatattccta ttccgggaca gcaagacaaa
     2161 gataatttcc aggagaccat ggaagcaatg cacataatgg gcttctccca tgaagagatt
     2221 ctgtcaatgc ttaaagtagt atcttcagtg ctacagtttg gaaatatttc tttcaaaaag
     2281 gagagaaata ctgatcaagc ttccatgcca gaaaatacag ttgcgcagaa gctctgccat
     2341 cttcttggga tgaatgtgat ggagtttact cgggccatcc tgactccccg gatcaaggtc
     2401 ggccgagact atgtgcaaaa agcccagacc aaagaacagg cagattttgc agtagaagca
     2461 ttggcaaaag ctacctatga gcggctcttt cgctggctcg ttcatcgcat caataaagct
     2521 ctggatagga ccaaacgtca gggagcatct ttcattggaa tcctggatat tgctggattt
     2581 gaaatttttg agctgaactc ctttgaacaa ctttgcatca actacaccaa tgagaagctg
     2641 cagcagctgt tcaaccacac catgtttatc ctagaacaag aggaatacca gcgcgaaggc
     2701 atcgagtgga acttcatcga tttcgggctg gatctgcagc catgcatcga cctaatagag
     2761 agacctgcga accctcctgg tgtactggcc cttttggatg aagaatgctg gttccctaaa
     2821 gccacagata aaacctttgt tgaaaaactg gttcaagagc aaggttccca ctccaagttt
     2881 cagaaacctc gacaattaaa agacaaagct gatttttgca ttatacatta tgcagggaag
     2941 gtggactata aggcagatga gtggctgatg aagaatatgg accccctgaa tgacaacgtg
     3001 gccacccttt tgcaccagtc atcagacaga tttgtggcag agctttggaa agatgtggac
     3061 cgtatcgtgg gtctggatca agtcactggt atgactgaga cagcttttgg ctccgcatat
     3121 aaaaccaaga agggcatgtt tcgtaccgtt gggcaactct acaaagaatc tctcaccaag
     3181 ctgatggcaa ctctccgaaa caccaaccct aactttgttc gttgtatcat tccaaatcac
     3241 gagaagaggg ctggaaaatt ggatccacac ctagtcctag atcagcttcg ctgtaatggt
     3301 gtcctggaag ggatccgaat ctgtcgccag ggcttcccta accgaatagt tttccaggaa
     3361 ttcagacaga gatatgagat cctaactcca aatgctattc ctaaaggttt tatggatggt
     3421 aaacaggcct gtgaacgaat gatccgggct ttagaattgg acccaaactt gtacagaatt
     3481 ggacagagca agatattttt cagagctgga gttctggcac acttagagga agaaagagat
     3541 ttaaaaatca ccgatatcat tatcttcttc caggccgttt gcagaggtta cctggccaga
     3601 aaggcctttg ccaagaagca gcagcaacta agtgccttaa aggtcttgca gcggaactgt
     3661 gccgcgtacc tgaaattacg gcactggcag tggtggcgag tcttcacaaa ggtgaagccg
     3721 cttctacaag tgactcgcca ggaggaagaa cttcaggcca aagatgaaga gctgttgaag
     3781 gtgaaggaga agcagacaaa ggtggaagga gagctggagg agatggagcg gaagcaccag
     3841 cagcttttag aagagaagaa tatccttgca gaacaactac aagcagagac tgagctcttt
     3901 gctgaagcag aagagatgag ggcaagactt gctgctaaaa agcaggaatt agaagagatt
     3961 ctacatgact tggagtctag ggttgaagaa gaagaagaaa gaaaccaaat cctccaaaat
     4021 gaaaagaaaa aaatgcaagc acatattcag gacctggaag aacagctaga cgaggaggaa
     4081 ggggctcggc aaaagctgca gctggaaaag gtgacagcag aggccaagat caagaagatg
     4141 gaagaggaga ttctgcttct cgaggaccaa aattccaagt tcatcaaaga aaagaaactc
     4201 atggaagatc gcattgctga gtgttcctct cagctggctg aagaggaaga aaaggcgaaa
     4261 aacttggcca aaatcaggaa taagcaagaa gtgatgatct cagatctaga agaacgctta
     4321 aagaaggaag aaaagactcg tcaggaactg gaaaaggcca aaagaaaact cgacggggag
     4381 acgaccgacc tgcaggacca gatcgcagag ctgcaggcgc agattgatga gctcaagctg
     4441 cagctggcca agaaggagga ggagctgcag ggcgcactgg ccagaggtga tgatgaaaca
     4501 ctccataaga acaatgccct taaagttgtg cgagagctac aagcccaaat tgctgaactt
     4561 caggaagact ttgaatccga gaaggcttca cggaacaagg ccgaaaagca gaaaagggac
     4621 ttgagtgagg aactggaagc tctgaaaaca gagctggagg acacgctgga caccacggca
     4681 gcccagcagg aactacgtac aaaacgtgaa caagaagtgg cagagctgaa gaaagctctt
     4741 gaggaggaaa ctaagaacca tgaagctcaa atccaggaca tgagacaaag acacgcaaca
     4801 gccctggagg agctctcaga gcagctggaa caggccaagc ggttcaaagc aaatctagag
     4861 aagaacaagc agggcctgga gacagataac aaggagctgg cgtgtgaggt gaaggtcctg
     4921 cagcaggtca aggctgagtc tgagcacaag aggaagaagc tcgacgcgca ggtccaggag
     4981 ctccatgcca aggtctctga aggcgacagg ctcagggtgg agctggcgga gaaagcaagt
     5041 aagctgcaga atgagctaga taatgtctcc acccttctgg aagaagcaga gaagaagggt
     5101 attaaatttg ctaaggatgc agctagtctt gagtctcaac tacaggatac acaggagctt
     5161 cttcaggagg agacacgcca gaaactaaac ctgagcagtc ggatccggca gctggaagag
     5221 gagaagaaca gtcttcagga gcagcaggag gaggaggagg aggccaggaa gaacctggag
     5281 aagcaagtgc tggccctgca gtcccagttg gctgatacca agaagaaagt agatgacgac
     5341 ctgggaacaa ttgaaagtct ggaagaagcc aagaagaagc ttctgaagga cgcggaggcc
     5401 ctgagccagc gcctggagga gaaggcactg gcgtatgaca aactggagaa gaccaagaac
     5461 cgcctgcagc aggagctgga cgacctcacg gtggacctgg accaccagcg ccaggtcgcc
     5521 tccaacttgg agaagaagca gaagaagttt gaccagctgt tagcagaaga gaagagcatc
     5581 tctgctcgct atgccgaaga gcgggaccgg gccgaagccg aggccagaga gaaagaaacc
     5641 aaagccctgt cactggcccg ggccctcgag gaagccctgg aggccaagga ggagtttgag
     5701 aggcagaaca agcagctccg agcagacatg gaagacctca tgagctccaa agatgatgtg
     5761 ggaaaaaacg ttcacgaact tgaaaaatcc aaacgggccc tagagcagca ggtggaggaa
     5821 atgaggaccc agctggagga gctggaagac gaactccagg ccacggaaga tgccaagctt
     5881 cgtctggagg tcaacatgca ggccatgaag gcgcagttcg agagagacct gcaaaccagg
     5941 gatgagcaga atgaagagaa gaagcggctg ctgatcaaac aggtgcggga gctcgaggcg
     6001 gagctggagg atgagaggaa acagcgggcg cttgctgtag cttcaaagaa aaagatggag
     6061 atagacctga aggacctcga agcccaaatc gaggctgcga acaaagctcg ggatgaggtg
     6121 attaagcagc tccgcaagct ccaggctcag atgaaggatt accaacgtga attagaagaa
     6181 gctcgtgcat ccagagatga gatttttgct caatccaaag agagtgaaaa gaaattgaag
     6241 agtctggaag cagaaatcct tcaattgcag gaggaacttg cctcatctga gcgagcccgc
     6301 cgacacgccg agcaggagag agatgagctg gcggacgaga tcaccaacag cgcctctggc
     6361 aagtccgcgc tgctggatga gaagcggcgt ctggaagctc ggatcgcaca gctggaggag
     6421 gagctggaag aggagcagag caacatggag ctgctcaacg accgcttccg caagaccact
     6481 ctacaggtgg acacactgaa cgccgagcta gcagccgagc gcagcgccgc ccagaagagt
     6541 gacaatgcac gccagcaact ggagcggcag aacaaggagc tgaaggccaa gctgcaggaa
     6601 ctcgagggtg ctgtcaagtc taagttcaag gccaccatct cagccctgga ggccaagatt
     6661 gggcagctgg aggagcagct tgagcaggaa gccaaggaac gagcagccgc caacaaatta
     6721 gtccgtcgca ctgagaagaa gctgaaagaa atcttcatgc aggttgagga tgagcgtcga
     6781 cacgcggacc agtataaaga gcagatggag aaggccaacg ctcggatgaa gcagcttaaa
     6841 cgccagctgg aggaagcaga agaagaagcg acgcgtgcca acgcatctcg gcgtaaactc
     6901 cagcgggaac tggatgatgc caccgaggcc aacgagggcc tgagccgcga ggtcagcacc
     6961 ctgaagaacc ggctgaggcg gggtggcccc atcagcttct cttccagccg atctggccgg
     7021 cgccagctgc accttgaagg agcttccctg gagctctccg acgatgacac agaaagtaag
     7081 accagtgatg tcaacgagac gcagccaccc cagtcagagt aaagttgcag gaagccagag
     7141 gaggcaatac agtgggacag ttaggaatgc acccggggcc tcctgcagat ttcggaaatt
     7201 ggcaagctac gggattcctt cctgaaagat caactgtgtc ttaaggctct ccagcctatg
     7261 catactgtat cctgcttcag acttaggtac aattgctccc ctttttatat atagacacac
     7321 acaggacaca tatattaaac agattgtttc atcattgcat ctattttcca tatagtcatc
     7381 aagagaccat tttataaaac atggtaagac cctttttaaa acaaactcca ggcccttggt
     7441 tgcgggtcgc tgggttattg gggcagcgcc gtggtcgtca ctcagtcgct ctgcatgctc
     7501 tctgtcatac agacaggtaa cctagttctg tgttcacgtg gcccccgacg actcagccac
     7561 atcaagtctc ctagaccact gtggactcta aactgcactt gtctctctca tttccttcaa
     7621 ataatgatca atgctatttc agtgagcaaa ctgtgaaagg ggctttggaa agagtaggag
     7681 gggtgggctg gatcggaagc aacacccatt tggggttacc atgtccatcc cccaaggggg
     7741 gccctccccc tcgagtcgat ggtgtcccgc atctactcat gtgaactggc cttggcgagg
     7801 gctggtctgt gcatagaagg gatagtggcc acactgcagc tgaggcccca ggtggcagcc
     7861 atggatcatg tagacttcca gatggtctcc cgaaccgcct ggctctgccg gcgccctcct
     7921 cacgtcagga gcaagcagcc gtggacccct aagccgagct ggtggaaggc ccctccccgt
     7981 cgccagccgg gccctcatgc tgaccttgca aattcagccg ctgctttgag cccaaaatgg
     8041 gaatattggt tttgtgtccg aggcttgttc caagtttgtc aatgaggttt atggagcctc
     8101 cagaacagat gccatcttcc tgaatgttga catgccagtg ggtgtgactc cttcattttt
     8161 ccttctccct tccctttgga cagtgttaca gtgaacactt agcatcctgt ttttggttgg
     8221 tagttaagca aactgacatt acggaaagtg ccttagacac tacagtacta agacaatgtt
     8281 gaatatatca ttcgcctcta taacaattta atgtattcag ttttgactgt gcttcatatc
     8341 atgtacctct ctagtcaaag tggtattaca gacattcagt gacaatgaat cagtgttaat
     8401 tctaaatcct tgatcctctg caatgtgctt gaaaacacaa accttttggg ttaaaagctt
     8461 taacatctat taggaagaat ttgtcctgtg ggtttggaat cttggatttt ccccctttat
     8521 gaactgtact ggctgttgac caccagacac ctgaccgcaa atatcttttc ttgtattccc
     8581 atatttctag acaatgattt ttgtaagaca atgcggccgc gaattcctgc agcccggggg
     8641 atccactagt tctagagcgg ccgccaccgc gggcccggga tccaccggat ctagataact
     8701 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     8761 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     8821 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8881 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg
     8941 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     9001 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     9061 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     9121 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     9181 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     9241 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     9301 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     9361 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     9421 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9481 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     9541 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9601 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9721 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9781 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9841 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     9901 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     9961 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
    10021 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
    10081 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    10141 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    10201 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    10261 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    10321 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    10381 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
    10441 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    10501 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    10561 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
    10621 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    10681 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
    10741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
    10801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    10861 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
    10921 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
    10981 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
    11041 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
    11101 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
    11161 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    11221 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    11281 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    11341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    11401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    11461 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    11521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11701 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11761 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11821 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11881 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11941 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    12001 ccgtattacc gc
//
