LOCUS       sequence-155967-       10382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Wei Q, Adelstein RS
  TITLE     Conditional expression of a truncated fragment of nonmuscle myosin 
            II-A alters cell shape but not cytokinesis in HeLa cells.
  JOURNAL   Mol Biol Cell. 2000 Oct . 11(10):3617-27.
  PUBMED    11029059
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2000 Oct . 11(10):3617-27."
COMMENT     Sequence Label: pTRE-GFP-NMHC II-A
FEATURES             Location/Qualifiers
     source          1..10382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..97
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    163..181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        256..294
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     291..315
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      394..403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(400..402,403..405,406..1116)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(400..402,403..405,406..1116)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(445..466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(706..725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1053..1074
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7177..7298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7214..7233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7268..7287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7305..7760)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7442..7463)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7654..7673
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7787..7891
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7893..8250
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7893..8084
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7916..7936)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8101..8236
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8285..9079
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8285..9079
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8339..8358)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8949..8968
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9267..9286)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9311..9358
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9687..10275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10176..10195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatcg tcctcgagtt taccactccc tatcagtgat agagaaaagt
       61 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      121 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      181 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
      241 cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      361 cgggaccgat ccagcctccg agcgctaccg gtcgccacca tggtgagcaa gggcgaggag
      421 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      481 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      541 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      601 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      661 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      721 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      781 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      841 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      901 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      961 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1021 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1081 gccgggatca ctctcggcat ggacgagctg tacaagtact cagatctcga gctcaagctt
     1141 atggcacagc aagctgccga taagtatctc tatgtggata aaaacttcat caacaatccg
     1201 ctggcccagg ccgactgggc tgccaagaag ctggtatggg tgccttccga caagagtggc
     1261 tttgagccag ccagcctcaa ggaggaggtg ggcgaagagg ccatcgtgga gctggtggag
     1321 aatgggaaga aggtgaaggt gaacaaggat gacatccaga agatgaaccc gcccaagttc
     1381 tccaaggtgg aggacatggc agagctcacg tgcctcaacg aagcctcggt gctgcacaac
     1441 ctcaaggagc gttactactc agggctcatc tacacctatt caggcctgtt ctgtgtggtc
     1501 atcaatcctt acaagaacct gcccatctac tctgaagaga ttgtggaaat gtacaagggc
     1561 aagaagaggc acgagatgcc ccctcacatc tatgccatca cagacaccgc ctacaggagt
     1621 atgatgcaag accgagaaga tcaatccatc ttgtgcactg gtgaatctgg agctggcaag
     1681 acggagaaca ccaagaaggt catccagtat ctggcgtacg tggcgtcctc gcacaagagc
     1741 aagaaggacc agggcgagct ggagcggcag ctgctgcagg ccaaccccat cctggaggcc
     1801 ttcgggaacg ccaagaccgt gaagaatgac aactcctccc gcttcggcaa attcattcgc
     1861 atcaactttg atgtcaatgg ctacattgtt ggagccaaca ttgagactta tcttttggag
     1921 aaatctcgtg ctatccgcca agccaaggaa gaacggacct tccacatctt ctattatctc
     1981 ctgtctgggg ctggagagca cctgaagacc gatctcctgt tggagccgta caacaaatac
     2041 cgcttcctgt ccaatggaca cgtcaccatc cccgggcagc aggacaagga catgttccag
     2101 gagaccatgg aggccatgag gattatgggc atcccagaag aggagcaaat gggcctgctg
     2161 cgggtcatct caggggttct tcagctcggc aacatcgtct tcaagaagga gcggaacact
     2221 gaccaggcgt ccatgcccga caacacagct gcccaaaagg tgtcccatct cttgggtatc
     2281 aatgtgaccg atttcaccag aggaatcctc accccgcgca tcaaggtggg acgggattac
     2341 gtccagaagg cgcagactaa agagcaggct gactttgcca tcgaggcctt ggccaaggcg
     2401 acctatgagc ggatgttccg ctggctggtg ctgcgcatca acaaggctct ggacaagacc
     2461 aagaggcagg gcgcctcctt catcgggatc ctggacattg ccggcttcga gatctttgat
     2521 ctgaactcgt ttgagcagct gtgcatcaat tacaccaatg agaagctgca gcagctcttc
     2581 aaccacacca tgttcatcct ggagcaggag gagtaccagc gcgagggcat cgagtggaac
     2641 ttcatcgact ttggcctcga cctgcagccc tgcatcgacc tcattgagaa gccagcaggc
     2701 cccccgggca ttctggccct gctggacgag gagtgctggt tccccaaagc caccgacaag
     2761 agcttcgtgg agaaggtgat gcaggagcag ggcacccacc ccaagttcca gaagcccaag
     2821 cagctgaagg acaaagctga tttctgcatt atccactatg ccggcaaggt ggattacaaa
     2881 gctgacgagt ggctgatgaa gaacatggat cccctgaatg acaacatcgc cacactgctc
     2941 caccagtcct ctgacaagtt tgtctcggag ctgtggaagg atgtggaccg catcatcggc
     3001 ctggaccagg tggccggcat gtcggagacc gcactgcccg gggccttcaa gacgcggaag
     3061 ggcatgttcc gcactgtggg gcagctttac aaggagcagc tggccaagct gatggctacg
     3121 ctgaggaaca cgaaccccaa ctttgtccgc tgcatcatcc ccaaccacga gaagaaggcc
     3181 ggcaagctgg acccgcatct cgtgctggac cagctgcgct gcaacggtgt tctcgagggc
     3241 atccgtatct gccgccaggg cttccccaac agggtggtct tccaggagtt tcggcagaga
     3301 tatgagatcc tgactccaaa ctccattccc aagggtttca tggacgggaa gcaggcgtgc
     3361 gtgctcatga taaaagccct ggagctcgac agcaatctgt accgcattgg ccagagcaaa
     3421 gtcttcttcc gtgccggtgt gctggcccac ctggaggagg agcgagacct gaagatcacc
     3481 gacgtcatca tagggttcca ggcctgctgc aggggctacc tggccaggaa agcatttgcc
     3541 aagcggcagc agcagcttac cgccatgaag gtcctccagc ggaactgcgc tgcctacctg
     3601 aagctgcgga actggcagtg gtggcggctc ttcaccaagg tcaagccgct gctgcaggtg
     3661 agccggcagg aggaggagat gatggccaag gaggaggagc tggtgaaggt cagagagaag
     3721 cagctggctg cggagaacag gctcacggag atggagacgc tgcagtctca gctcatggca
     3781 gagaaattgc agctgcagga gcagctccag gcagaaaccg agctgtgtgc cgaggctgag
     3841 gagctccggg cccgcctgac cgccaagaag caggaattag aagagatctg ccatgaccta
     3901 gaggccaggg tggaggagga ggaggagcgc tgccagcacc tgcaggcgga gaagaagaag
     3961 atgcagcaga acatccagga gcttgaggag cagctggagg aggaggagag cgcccggcag
     4021 aagctgcagc tggagaaggt gaccaccgag gcgaagctga aaaagctgga ggaggagcag
     4081 atcatcctgg aggaccagaa ctgcaagctg gccaaggaaa agaaactgct ggaagacaga
     4141 atagctgagt tcaccaccaa cctcacagaa gaggaggaga aatctaagag cctcgccaag
     4201 ctcaagaaca agcatgaggc aatgatcact gacttggaag agcgcctccg cagggaggag
     4261 aagcagcgac aggagctgga gaagacccgc cggaagctgg agggagactc cacagacctc
     4321 agcgaccaga tcgccgagct ccaggcccag atcgcggagc tcaagatgca gctggccaag
     4381 aaagaggagg agctccaggc cgccctggcc agagtggaag aggaagctgc ccagaagaac
     4441 atggccctca agaagatccg ggagctggaa tctcagatct ctgaactcca ggaagacctg
     4501 gagtctgagc gtgcttccag gaataaagct gagaagcaga aacgggacct tggggaagag
     4561 ctagaggcgc tgaaaacaga gttggaggac acgctggatt ccacagctgc ccagcaggag
     4621 ctcaggtcaa aacgtgagca ggaggtgaac atcctgaaga agaccctgga ggaggaggcc
     4681 aagacccacg aggcccagat ccaggagatg aggcagaagc actcacaggc cgtggaggag
     4741 ctggcggagc agctggagca gacgaagcgg gtgaaagcaa acctcgagaa ggcaaagcag
     4801 actctggaga acgagcgggg ggagctggcc aacgaggtga aggtgctgct gcagggcaaa
     4861 ggggactcgg agcacaagcg caagaaagtg gaggcgcagc tgcaggagct gcaggtcaag
     4921 ttcaacgagg gagagcgcgt gcgcacagag ctggccgaca aggtcaccaa gctgcaggtg
     4981 gagctggaca acgtgaccgg gcttctcagc cagtccgaca gcaagtccag caagctcacc
     5041 aaggacttct ccgcgctgga gtcccagctg caggacactc aggagctgct gcaggaggag
     5101 aaccggcaga agctgagcct gagcaccaag ctcaagcagg tggaggacga gaagaattcc
     5161 ttccgggagc agctggagga ggaggaggag gccaagcaca acctggagaa gcagatcgcc
     5221 accctccatg cccaggtggc cgacatgaaa aagaagatgg aggacagtgt ggggtgcctg
     5281 gaaactgctg aggaggtgaa gaggaagctc cagaaggacc tggagggcct gagccagcgg
     5341 cacgaggaga aggtggccgc ctacgacaag ctggagaaga ccaagacgcg gctgcagcag
     5401 gagctggacg acctgctggt ggacctggac caccagcgcc agagcgcgtg caacctggag
     5461 aagaagcaga agaagtttga ccagctcctg gcggaggaga agaccatctc tgccaagtat
     5521 gcagaggagc gcgaccgggc tgaggcggag gcccgagaga aggagaccaa ggctctgtcg
     5581 ctggcccggg ccctggagga agccatggag cagaaggcgg agctggagcg gctcaacaag
     5641 cagttccgca cggagatgga ggaccttatg agctccaagg atgatgtggg caagagtgtc
     5701 cacgagctgg agaagtccaa gcgggcccta gagcagcagg tggaggagat gaagacgcag
     5761 ctggaagagc tggaggacga gctgcaggcc accgaagatg ccaagctgcg gttggaggtc
     5821 aacctgcagg ccatgaaggc ccagttcgag cgggacctgc agggccggga cgagcagagc
     5881 gaggagaaga agaagcagct ggtcagacag gtgcgggaga tggaggcaga gctggaggac
     5941 gagaggaagc agcgctcgat ggcagtggcc gcccggaaga agctggagat ggacctgaag
     6001 gacctggagg cgcacatcga ctcggccaac aagaaccggg acgaagccat caaacagctg
     6061 cggaagctgc aggcccagat gaaggactgc atgcgcgagc tggatgacac ccgcgcctct
     6121 cgtgaggaga tcctggccca ggccaaagag aacgagaaga agctgaagag catggaggcc
     6181 gagatgatcc agttgcagga ggaactggca gccgcggagc gtgccaagcg ccaggcccag
     6241 caggagcggg atgagctggc tgacgagatc gccaacagca gcggcaaagg agccctggcg
     6301 ttagaggaga agcggcgtct ggaggcccgc atcgcccagc tggaggagga gctggaggag
     6361 gagcagggca acacggagct gatcaacgac cggctgaaga aggccaacct gcagatcgac
     6421 cagatcaaca ccgacctgaa cctggagcgc agccacgccc agaagaacga gaatgctcgg
     6481 cagcagctgg aacgccagaa caaggagctt aaggtcaagc tgcaggagat ggagggcact
     6541 gtcaagtcca agtacaaggc ctccatcacc gccctcgagg ccaagattgc acagctggag
     6601 gagcagctgg acaacgagac caaggagcgc caggcagcct gcaaacaggt gcgtcggacc
     6661 gagaagaagc tgaaggatgt gctgctgcag gtggatgacg agcggaggaa cgccgagcag
     6721 tacaaggacc aggccgacaa ggcatctacc cgcctgaagc agctcaagcg gcagctggag
     6781 gaggccgaag aggaggccca gcgggccaac gcctcccgcc ggaaactgca gcgcgagctg
     6841 gaggacgcca ctgagacggc cgatgccatg aaccgcgaag tcagctccct aaagaacaag
     6901 ctcaggcgcg gggacctgcc gtttgtcgtg ccccgccgaa tggcccggaa aggcgccggg
     6961 gatggctccg acgaagaggt agatggcaaa gcggatgggg ctgaggccaa acctgccgaa
     7021 taagtcgact ctaatcacta gtgcggccga tccaccggat ctagataact gatcataatc
     7081 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     7141 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     7201 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7261 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     7321 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     7381 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     7441 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     7501 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     7561 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     7621 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     7681 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     7741 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7861 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     7921 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7981 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8041 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8101 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8161 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8221 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     8281 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     8341 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     8401 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     8461 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     8521 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     8581 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     8641 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     8701 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     8761 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     8821 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     8881 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8941 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     9001 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     9061 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     9121 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     9181 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9241 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     9301 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     9361 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     9421 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     9481 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     9541 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9601 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9661 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9721 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9781 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9841 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9901 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9961 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10021 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10081 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10141 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10201 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10261 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10321 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10381 gc
//
