LOCUS       sequence-155974-       10151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector, Cerulean-2A-sHRPbNLG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1
FEATURES             Location/Qualifiers
     source          1..10151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1072..1375
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1378..1653
                     /label=chicken beta-actin promoter
     intron          1654..2662
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2586..2608
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2670..2689
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2729..2753
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2797..2806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2803..2805,2806..2808,2809..3519)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2848..2869)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3109..3128)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3456..3477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4153..4194
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      4656..4665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6621..6645)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6736..6755)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6801..6856
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6855..6874)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   7477..7511
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8002..8018)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8002..8018)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8015..8037)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8026..8042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8050..8056,8057..8074,8075..8080))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8095..8116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8233..8250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8404..8992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8484..8503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9163..9954,9955..10023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9786..9805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10024..10128)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
      961 tctataaagt aacaaaactt ttatcgaatt cctgcagccc gggggatcca ctagttatta
     1021 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     1081 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     1141 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     1201 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     1261 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     1321 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg
     1381 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     1441 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
     1501 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
     1561 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
     1621 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
     1681 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     1741 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     1801 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
     1861 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     1921 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1981 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     2041 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     2101 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     2161 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     2221 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     2281 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2341 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     2401 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     2461 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     2521 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     2581 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     2641 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2701 ttttggcaaa gaattcctcg aggatatcac aagtttgtac aaaaaagcag gctccgaatt
     2761 cgcccttcgt aactataacg gtcctaaggt agcgaagcca ccatggtgag caagggcgag
     2821 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2881 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2941 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     3001 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     3061 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3121 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3181 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3241 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     3301 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     3361 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     3421 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3481 gccgccggga tcactctcgg catggacgag ctgtacaaga ctagtagtac taacttcgac
     3541 ctgctgaagc tggccggcga cgtggagagc aatcctggac ctaattttga tctcctcaaa
     3601 ctcgctggag atgtcgaatc caatcctgga cctaactttg acttgttgaa gttggcaggg
     3661 gacgttgagt ctaacccagg gccagctagc gatatcgcgg ccgcgatggc acttcccaga
     3721 tgcatgtggc caaattatgt ttggagagct atgatggcat gtgtggtcca caggggatcc
     3781 ggtgccccat tgactctctg cttgttggga tgtttgctac agacttttca cgtactctct
     3841 caaaagaccg gtaacttgtc tgcactggtt gactttgacc tgcgcacccc aaccatcttc
     3901 gacaataagt attacgtcaa tctggaggaa cagaagggcc tgattcagtc cgaccaggag
     3961 ctgttcagtt ccccagatgc taccgacact attcctcttg tgcgcagctt cgctaatagc
     4021 acacaaacct ttttcaatgc tttcgtggaa gctatggatc ggatgggaaa tataacgccc
     4081 ctgacgggta cgcagggaca gataagacgg aactgtaggg tcgtgaacag caactctggc
     4141 tcgggctcgg gcggcaagcc catccccaac cccctgctgg gcctggacag cacccaattg
     4201 ttggatgatg tagacccatt ggttactact aactttggca agattagggg aattaagaaa
     4261 gaactcaata atgaaatttt gggtcctgtc attcagtttc ttggggttcc atatgccgct
     4321 ccaccaacag gagaacatcg tttccagcct ccagaaccac catctccctg gtctgacatc
     4381 cggaacgcca ctcagtttgc tcctgtatgt ccccagaata tcattgatgg cagattgcct
     4441 gaagttatgc ttcctgtgtg gttcactaat aacttggatg tggtttcatc atacgtccaa
     4501 gaccagagtg aagactgtct atacttaaac atctatgtcc caactgaaga tggtcccctt
     4561 acaaagaaac acacagatga tttaggtgat aatgacggtg ctgaagatga agatattcgg
     4621 gacagtgggg gtcccaaacc agtgatggtg tacatccatg gcggctctta catggaaggt
     4681 actggaaatc tgtatgatgg gagtgtcttg gcaagctatg gcaatgtgat cgtcatcaca
     4741 gtcaactatc ggcttggggt acttggcttc ttgagcacag gggatcaggc tgccaaagga
     4801 aactacgggc tccttgacct catccaggcc ctaagatgga ccagcgagaa cattgggttc
     4861 tttggtggtg accccttgcg aatcactgtg tttggatcag gcgctggggg ttcatgtgtc
     4921 aacctgctga ctttatccca ttattctgaa ggtaaccgtt ggagcaattc aaccaaagga
     4981 ctttttcaac gagcaatagc tcagagtgga acagcccttt ccagctgggc tgttagtttc
     5041 cagcctgcaa aatacgctag aattctggcc acaaaagttg gctgcaatgt ttcagataca
     5101 gtagagttag tagaatgcct gcagaagaag ccttacaaag aacttgttga tcaagatgtt
     5161 caaccagccc gataccacat agcctttgga cctgtgatcg atggtgatgt aataccagat
     5221 gaccctcaga tactgatgga acaaggagag ttcctcaact atgatataat gttgggagtt
     5281 aaccaagggg aagggttgaa gtttgtcgaa aatatagtag atagtgatga tggtgtatca
     5341 gccagtgatt tcgactttgc tgtttctaat tttgttgata atttatatgg atatcctgaa
     5401 ggcaaagatg ttttgagaga aaccattaaa ttcatgtata ctgactgggc tgatcgccat
     5461 aatcctgaaa ctagaaggaa gacattgttg gctttgttta cggaccatca atgggtagca
     5521 cctgctgtgg ccacagcaga ccttcactcg aactttggct cacctacata cttctatgcc
     5581 ttttatcatc attgccaaac agatcaagtt ccagcttggg ctgatgcagc tcatggggat
     5641 gaggttccct atgtgttggg aatccccatg attggcccta cagagttatt tccttgcaat
     5701 ttctccaaga atgatgtgat gttgagtgca gtagtaatga catactggac gaattttgct
     5761 aaaactggtg acccaaatca accagttcct caagacacaa aattcatcca taccaaaccc
     5821 aaccgctttg aagaagtagc atggaccaga tattcccaga aagaccagct ttatctccat
     5881 attggattaa aaccgagagt taaagagcat tacagagcca ataaggtaaa tctctggctg
     5941 gagctggtac ctcatctgca taatctcaat gacatttctc agtatacctc gacaacaact
     6001 aaagtgccat ccacggacat cactctcaga cctacaagga agaattccac accagtcaca
     6061 tcagcctttc ccactgccaa acaggatgat cccaagcaac aaccaagccc cttctcggtg
     6121 gatcagaggg actactccac agagctaagt gtcactatcg cagtgggggc ctctctgctg
     6181 tttctcaaca tcttggcttt tgcagccctg tactacaaga aggataagag gagacatgat
     6241 gtccaccgga ggtgcagccc tcagcgcacg accaccaacg acctaaccca tgctccagaa
     6301 gaggaaatta tgtctctcca aatgaagcac actgacttgg atcacgagtg tgagtccatc
     6361 catccacatg aggtggttct tcggaccgcc tgtcccccag attatactct agctatgagg
     6421 aggtcacctg atgatattcc actaatgaca cctaacacca tcacaatgat tcccaacact
     6481 ataccaggga ttcagccctt acatacattc aacacattta ctggaggaca gaataataca
     6541 ctgccccatc cccacccaca cccccattca cattcaacaa ccagggtata gggcgcgccg
     6601 ggcggccaag ggcgaattcg acccagcttt cttgtacaaa gtggtgattc gcggccgcac
     6661 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     6721 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     6781 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     6841 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     6901 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt
     6961 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     7021 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     7081 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     7141 tactcccagt catagctgtc cctcttctct tatgaagatc cctcgacctg cagcccaagc
     7201 ttgatatcta taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa
     7261 taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt
     7321 cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc
     7381 acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg
     7441 cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta
     7501 tctttctagg gttaatctag ctgcatcagg atcatatcgt cgggtctttt ttccggctca
     7561 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     7621 gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg
     7681 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg
     7741 gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt
     7801 tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact
     7861 gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg
     7921 acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     7981 ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8041 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8821 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9301 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10141 aaaagtgcca c
//
