LOCUS       sequence-155977-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APEX in the mitochondrial intermembrane space..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Hung V, Zou P, Rhee HW, Udeshi ND, Cracan V, Svinkina T, Carr SA, 
            Mootha VK, Ting AY
  TITLE     Proteomic mapping of the human mitochondrial intermembrane space in 
            live cells via ratiometric APEX tagging.
  JOURNAL   Mol Cell. 2014 Jul 17;55(2):332-41. doi: 
            10.1016/j.molcel.2014.06.003. Epub 2014 Jul 4.
  PUBMED    25002142
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.06.003"; journalName: "Mol Cell"; date: 
            "2014-07-17- 17"; volume: "55"; issue: "2"; pages: "332-41"
COMMENT     Sequence Label: IMS-APEX2
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..106)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    108..132
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(140..886)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgcca ccggttagcg ctagctcatt
       61 actacgtaga atcgagaccg aggagagggt tagggatagg cttaccaacc actttgtaca
      121 agaaagctgg gtcttatcag gcatcagcaa acccaagctc ggaaagcttt tggtgagcct
      181 cagcgtaatc agcaaagaag gcatcttcgt ccgctgcata tttatcaacg agagggcgga
      241 atacagggtc agacaaaaga gccttgtcag aaggtagctg aaggagacct tccttctcac
      301 cactcaacaa ctccgtgaag tatgagttgt cgaaaataag aggattagag gtccagggac
      361 cctcaaatcc agaacgctcc ttgtgtgcag ctccaatagt gtgaccccca gatagagcaa
      421 cgatatcttg gtcagtaagc cccatagctt tgccaaacac atctctcaaa tggtcagaac
      481 ccttagtggg atcgggcaag cgaccctctg gtggtggctc aggcttgtcc tctcttccag
      541 ggtggaatgg aaccttaggt ccacccgtga cctcaacggc aacaacgcca gccaactggt
      601 agaaatcggc gtagctcaaa atagggaact ccgccttgag tggctccaaa agcctaacag
      661 cgatgtcaag accgttgtta gcgctgtgag ccagttcggc agggtgcttg atggttccga
      721 agggtccacc ggtcttcgtg cccttgtcaa aggttccagc agagtggaat gccaaacgga
      781 gcattagagg agcgcatctc ttctcagcga tgaagcctct gagcttcttc ttcgccttct
      841 caacggcgtc ctggtaatca gcactcacag ttgggtaaga ctttccggtg ctgtccaggc
      901 ccagcag
//
