LOCUS       sequence-155997-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE77
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             121..141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      157..409
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        502..681
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt
       61 acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac
      121 cccaagaaga agaggaaggt gtgagcttgt caagcagatc gttcaaacat ttggcaataa
      181 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
      241 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
      301 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
      361 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg acgctactaa
      421 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
      481 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc cgcgggtttc
      541 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
      601 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
      661 attcccctcg gtatccaatt agagtctcat attcactctc aactcgatcg aggggatcta
      721 ccatgagccc agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg
      781 cggtctgcac catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc
      841 cgcaggaacc gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc
      901 tcgtcgccga ggtggacggc gag
//
