LOCUS       sequence-156011-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a CAG 
            promoter and loxP flanked stop cassette, for cloning into AsiSI or 
            Mlui.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-01-16"; volume: "16(1):4. doi"; pages: " 
            10.1186/s12896-016-0234-4"
COMMENT     Sequence Label: pR26 CAG AsiSI/MluI
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga tcaagcttat cgataccgtc
       61 gacggtatcg ataagctaga tgtcctgaaa tatttctatc agaacaaggt agtataaagc
      121 tggtaggtat acaaaacgct agactagttt ctatccctga cccttaatct gctagtatat
      181 ccgtaggaag ttgcttaagt gccactagta ccaacagcct ctgttagagt gaacagtaga
      241 ttttaatgaa gggccaataa agaccactta agactactga ctgaattaaa aatccaccca
      301 gtggagttag tattggttat agttatataa caaaatatcc aacttagcca ggcgtggtgg
      361 tgcacgcctt taatcccggc actcaggagg cagaggcagg cggatttctg agttcgaggc
      421 cagcctggtc tacaaagtga gttccaggac agccagggct atacagagaa accctgtctc
      481 gaaaaaccaa aaaaaaaaaa aaaaaggaaa aaaaatatgg cttgaaaaca tgctattgtt
      541 gtcaaaagga gatccaaaca agtatttctt tgaacaaaaa ttatgtagaa aacagggtga
      601 taaagaacaa gggcttttta aaaaaggcca gcattatttg agatatacaa gtgactccaa
      661 ttcaagccta aaggccactc aatgctcact aacagtgcta atcagaatca attccttagc
      721 ccagtcaaaa atagtcctga atatcttctg gataaacatg tcctccaaca tataaactag
      781 tatacactaa c
//
