LOCUS       sequence-156029-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cre recombinase alongside Cas9 and an 
            sgRNA cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Walter DM, Venancio OS, Buza EL, Tobias JW, Deshpande C, Gudiel AA, 
            Kim-Kiselak C, Cicchini M, Yates TJ, Feldser DM
  TITLE     Systematic in vivo inactivation of chromatin regulating enzymes 
            identifies Setd2 as a potent tumor suppressor in lung 
            adenocarcinoma.
  JOURNAL   Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 
            10.1158/0008-5472.CAN-16-2159.
  PUBMED    28202515
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2017 Feb 15. pii: canres.2159.2016. doi: 
            10.1158/0008-5472.CAN-16-2159."
COMMENT     Sequence Label: LentiCRISPRv2Cre
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             518..565
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             566..589
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             599..655
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             659..679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
       61 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
      121 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
      181 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
      241 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
      301 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
      361 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
      421 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
      481 cgagacacgg atcgacctgt ctcagctggg aggcgacaag cgacctgccg ccacaaagaa
      541 ggctggacag gctaagaaga agaaagatta caaagacgat gacgataagg gatccggcgc
      601 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgcc
      661 caagaagaag aggaaggtgt ccaatttact gaccgtacac caaaatttgc ctgcattacc
      721 ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca
      781 ggcgttttct gagcatacct gga
//
