LOCUS       sequence-156034-         560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSEVA331 with Biobrick multiple cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pSEVA331Bb
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..49
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    50..71
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(73..95)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      110..196
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttctgggatc tatcaacagg agtccaagct gcagcggccg ctactagtac tctagaagcg
       61 gccgcgaatt ctcctgtgtg aaattgttat ccgctttaat taaaggcatc aaataaaacg
      121 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      181 cctgagtagg acaaatccgc cgccctagac agctgggcgc gcccccccta cgggcttgct
      241 ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga tatgtggacg
      301 atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga
      361 caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct
      421 acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat
      481 caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg
      541 ccggttggac accaagtgga
//
