LOCUS       sequence-156035-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSEVA331 with Biobrick multiple cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pSEVA331Bb
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..68
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     misc_feature    70..91
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    92..112
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      117..211
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        259..361
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(428..447)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agtctttcga ctgagccttt cgttttattt gatgccttta attaaagcgg ataacaattt
       61 cacacaggag aattcgcggc cgcttctaga gtactagtag cggccgctgc agcttggact
      121 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      181 ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa
      241 ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat
      301 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
      361 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      421 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      481 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      541 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
      601 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      661 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
      721 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
      781 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
      841 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
      901 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggct
//
