LOCUS       sequence-156036-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast two-hybrid expression vector, expresses Gal4BD fused to 
            Arabidopsis CRY2 (residues 1-535).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL
  TITLE     Optimized second-generation CRY2-CIB dimerizers and photoactivatable
            Cre recombinase.
  JOURNAL   Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.
  PUBMED    27065233
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063."
COMMENT     Sequence Label: Gal4BD-CRY2(535)
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(64..165)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(179..195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(179..195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(216..235)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     335..357
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(395..413)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gctctgcaag ccgcaaactt tcaccaatgg accagaacta cctgtgaaat taataacaga
       61 catactccaa gctgcctttg tgtgcttaat cacgtatact cacgtgctca atagtcacca
      121 atgccctccc tcttggccct ctccttttct tttttcgacc gaattaattc gtaatcatgt
      181 catagctgtt tcctgtcgat atggtgcact ctcagtacaa tctgctctga tgccgcatag
      241 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      301 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      361 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
      421 gttaatgtca tgataataat ggtttcttag gacggatcgc ttgcctgtaa cttacacgcg
      481 cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat
      541 gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa
      601 aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta
      661 ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa
      721 atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat
      781 acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct
      841 tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt
      901 ttaatttata tatttatatt aaaaaattta aattataatt atttt
//
