LOCUS       sequence-156043-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  create protein aggregates in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, 
            Wandless TJ
  TITLE     A method to rapidly create protein aggregates in living cells.
  JOURNAL   Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689.
  PUBMED    27229621
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-05-27"; volume: "7:11689. doi"; pages: " 
            10.1038/ncomms11689"
COMMENT     Sequence Label: Pvha6-AgDD
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..325)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
ORIGIN
        1 attcttccgg ttttagaagc tccacatcga agacgagagt ggcatgtggt gggatgatgc
       61 ctgggtgccc agtggcacca taggcataat ctggagatat agtcagtttg gctctctgac
      121 ccacactcat ctgggcaacc ccttcttccc agcctcggat cacctcctgc ttgcctagca
      181 taaacttaaa gggcttgttt ctgtcccggg aggaatcgac tttctttcca tcttcaagca
      241 tcccggtgta gtgcaccaca caggtctggc cgcgcttggg gaaggtgcgc ccgtctcctg
      301 gggagatggt ttccacctgc actccaatat ctagaccagc cagcttcagg gccagcatgg
      361 tggcggatcc tctagaggcg cgccgggtag gttttagtcg ccctgaaatt aaacaatttt
      421 ataacaaaaa tcatttgaaa tggacatcag aaagttagga aaatgcggaa aaataggaga
      481 aacaaaaaat aaagtgctgc agaactaaag actgaaaacc gaagacaatt ttttagttct
      541 tcattgtctt atcagtgctc cgctttgctg ctccgtcagg cacgtcaaag aagtcgatta
      601 tcaatatcag accgccacca atttttgtgt ggccggagct gctctggaat gatggatgat
      661 gtattgagaa atgaagatga agccttacaa gttcaattcg atatatacct gcaatgattc
      721 aattgaaaca atatatactg aatgactagg aaatgtgggt ggtggcaata cagataacat
      781 tgttggctca ttgtt
//
