LOCUS       sequence-156054-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of GAK. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: GAK_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca gcgggtccag gctccctggg cggtgcttcc ggccgcgacc
      181 agagtgactt cgtggggcag acggtggaac tgggcgagct gcggctgcgg gtgcggcggg
      241 tcctggccga aggagggttt gcatttgtgt atgaagctca agatgtgggg agtggcagag
      301 agtatgcatt aaagaggcta ttatccaatg aagaggaaaa gaacagagcc atcattcaag
      361 aagtttgctt catgaaaaag ctttccggcc acccgaacat tgtccagttt tgttctgcag
      421 cgtctatagg aaaagaggag tcagacacgg ggcaggctga gttcctcttg ctcacagagc
      481 tctgtaaagg gcagctggtg gaatttttga agaaaatgga atctcgaggc cccctttcgt
      541 gcgacacggt tctgaagatc ttctaccaga cgtgccgcgc cgtgcagcac atgcaccggc
      601 agaagccgcc catcatccac agggacctca aggttgagaa cttgttgctt agtaaccaag
      661 ggaccattaa gctgtgtgac tttggcagtg ccacgaccat ctcgcactac cctgactaca
      721 gctggagcgc ccagaggcga gccctggtgg aggaagagat cacgaggaat acaacaccaa
      781 tgtatagaac accagaa
//
