LOCUS       sequence-156058-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of PMYT1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: PMYT1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca caccagttgc aacctcgtcg tgtttctttt cgcggcgagg
      181 cttcagaaac cttgcagagc ccaggttatg atccgtcccg cccagaaagt ttctttcaac
      241 agtcgttcca acgcctcagc cgtctgggac atggttctta cggcgaagta ttcaaagtac
      301 gctccaaaga ggacggccgt ttgtatgcgg tgaaacgctc aatgagtccg tttcgtggcc
      361 ccaaagatcg cgcccgtaag ctggcggaag ttggctctca cgagaaagtc ggccagcatc
      421 cgtgttgcgt acgcctggag caagcctggg aagaaggcgg tattctgtac ctgcagacag
      481 agctgtgcgg cccttcactg caacagcact gtgaggcgtg gggtgcaagt ttgccagaag
      541 ctcaagtgtg gggttatctg cgtgacacct tgctggcctt ggcacatctg cattcgcagg
      601 gtttggttca cctggatgtc aagcccgcca acatcttcct gggccctcgt ggtcgttgta
      661 aactgggcga ttttggcctg ttggtggagt tgggcactgc gggtgctggt gaagtacaag
      721 aaggcgatcc gcgttacatg gcaccagaat tgctgcaggg ctcttatggt acagccgcgg
      781 atgttttcag cctgggcttg actattctgg aggtagcttg caatatggag ctgccccacg
      841 gcggcga
//
