LOCUS       sequence-156062-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of HASP. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: HASP_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             56..78
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             98..127
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             101..130
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             104..133
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             134..154
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggagagctc tttaattaag cggccgccct gcaggactcg agttctagaa ataattttgt
       61 ttaactttaa gaaggagata tacatatgaa atcttctcac catcaccatc accatcacca
      121 tcaccatcac catgaaaacc tgtacttcca atccaatgca ggagaatgta gtcagaaggg
      181 ccccgtacca ttctcgcact gcctccccac ggagaaattg caacgttgtg agaaaatcgg
      241 tgaaggcgtg tttggtgagg tatttcagac cattgccgac catactccgg tcgctatcaa
      301 gattatcgcg attgaaggcc ccgatctggt aaacggtagt caccaaaaga catttgagga
      361 aatcttgcca gagattatca tctccaagga actgtctttg ctgtcaggcg aggtgtgcaa
      421 tcgcacagaa ggtttcattg gcctgaacag tgttcattgt gtccagggtt cgtatcctcc
      481 gttgctgttg aaagcatggg accactacaa cagcactaaa ggctccgcca atgatcgtcc
      541 cgacttcttc aaggatgacc aactgttcat cgtgctggag tttgagttcg gcggcattga
      601 tttggaacag atgcgcacca aactctcttc attggctaca gcgaagagta tcctgcatca
      661 gttgaccgcc agcctggcag tagctgaggc ctccctgcgt tttgaacacc gcgacttgca
      721 ttggggtaac gtgctgttga agaaaacttc gctgaagaaa ctgcactata cattgaatgg
      781 caagtcgtca accattccaa gttgcggtct gcaagttagc atcattgatt acactttgtc
      841 ccgtctggag cgcgacggca tcgtcgtatt t
//
