LOCUS       sequence-156067-        1331 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 684, Emission: 708.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien
            RY
  TITLE     Mammalian expression of infrared fluorescent proteins engineered 
            from a bacterial phytochrome.
  JOURNAL   Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683.
  PUBMED    19423828
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-05-8- 8"; volume: "324"; issue: 
            "5928"; pages: "804-7"
COMMENT     Sequence Label: IFP1.4-N1
FEATURES             Location/Qualifiers
     source          1..1331
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    23..103
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..1076
                     /codon_start=1
                     /product="bacteriophytochrome-based infrared fluorescent 
                     protein (Shu et al., 2009)"
                     /label=IFP1.1
                     /translation="MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASTTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEA"
     polyA_signal    1198..1279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1235..1254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gccggctagt gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc
       61 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggctcggg
      121 accctctgcc attctttcca cctctgtacc tgggcggccc tgagattaca accgagaact
      181 gcgagagaga gcctatccac attcctgggt ccatccagcc acacggggct ctgctcacag
      241 ctgacggcca ctccggcgag gtgctccaag tgtccctgaa tgccgctacc ttcctgggcc
      301 aggagcctac tgtgctgcgg gggcagaccc tggctgccct gctccccgag cagtggccag
      361 ccctgcaggc agccctgccc ccaggatgtc cagatgccct ccaatacagg gccaccctcg
      421 actggccagc tgctgggcac ctcagcctga ctgtgcatcg ggtggctgaa ctcctgatcc
      481 tggagttcga acctaccgag gcctgggaca gcattggccc tcacgccctg aggaacgcca
      541 tgtttgctct ggaaagcgct ccaaacctgc gggctctggc cgaagtcgca acacaaactg
      601 tgagagaact gactggcttc gatcgggtga tgctgtacaa atttgcccct gacgccactg
      661 gagagatgat tgctgaggcc agacgggagg gcatgcaggc ttttctgggc cacaggtttc
      721 ccgcatccac cacccctgca caagctaggg ccctctacac aagacacctg ctccggctga
      781 ccgcagacac cagggctgca gcagtgcccc tcgaccccgt gctgaatccc cagacaaatg
      841 ctcctacacc tctgggcgga gctgtcctca gagctacatc cccaatgcac atgcagtacc
      901 tgaggaatat gggagtgggc tcctccctga gcgtcagcgt cgtggtcggc ggccagctgt
      961 ggggactgat tgtctgccac catcagacac cctacgtgct gccacccgat ctgcggacca
     1021 ccctggagga gctggggaga aagctgtccg ggcaggtgca gaggaaagaa gcctgagcgg
     1081 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1141 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1261 aaagcatttt tttcactgca ttcatttggg tggtcaaacc tcactcaagt gttatctctt
     1321 taagaggcct a
//
