LOCUS       sequence-156068-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of CDKL1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: CDKL1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca atggagaagt atgaaaaaat tgggaaaatt ggagaaggct
      181 cctatggagt tgttttcaaa tgtagaaaca gggacacggg tcagattgtg gccatcaaga
      241 agtttctgga atcagaagat gaccctgtca taaagaaaat tgcccttcgg gaaatccgaa
      301 tgctcaagca actcaagcat cccaaccttg ttaacctcct ggaagtcttc aggaggaaac
      361 ggaggcttca cctggtgttt gaatattgtg accacacagt tctccatgag ttggacagat
      421 accaaagagg ggtaccagaa catctcgtga agagcataac ttggcagaca ctgcaagctg
      481 taaatttttg ccataaacac aattgcatac atagagacgt gaagccagaa aatatcctca
      541 tcacgaaaca ttccgtgatt aagctttgtg actttggatt tgctcggctt ttgactggac
      601 cgagtgacta ctatgacgac gaagtggcta ccaggtggta ccgctcccct gagctgctgg
      661 tgggggacac gcagtacggc cccccggtgg atgtttgggc aattggctgt gtctttgctg
      721 agctgctgtc aggagtgcct ctgtggccag gaaaatcgga tgtggatcag ctgtatctga
      781 ttaggaagac cttgggggat ctcattccta ggcaccagca agtgtttagc acgaatcagt
      841 acttcag
//
