LOCUS       sequence-156071-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of BMPR2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: BMPR2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca atggaggcag cagcatccga accctctctt gatctagata
      181 atctgaaact gttggagctg attggccgag gtcgatatgg agcagtatat aaaggctcct
      241 tggatgagcg tccagttgct gtaaaagtgt tttcctttgc aaaccgtcag aattttatca
      301 acgaaaagaa catttacaga gtgcctttga tggaacatga caacattgcc cgctttatag
      361 ttggagatga gagagtcact gcagatggac gcatggaata tttgcttgtg atggagtact
      421 atcccaatgg atctttatgc aagtatttaa gtctccacac aagtgactgg gtaagctctt
      481 gccgtcttgc tcattctgtt actagaggac tggcttatct tcacacagaa ttaccacgag
      541 gagatcatta taaacctgca atttcccatc gagatttaaa cagcagaaat gtcctagtga
      601 aaaatgatgg aacctgtgtt attagtgact ttggactgtc catgaggctg actggaaata
      661 gactggtgcg cccaggggag gaagataatg cagccataag cgaggttggc actatcagat
      721 atatggcacc aga
//
