LOCUS       sequence-156073-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KC1G1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KC1G1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca agggttggca agaagatagg atgtgggaac ttcggagagc
      181 tcagattagg taaaaatctc tacaccaatg aatatgtagc aatcaaactg gaaccaataa
      241 aatcacgtgc tccacagctt catttagagt acagatttta taaacagctt ggcagtgcag
      301 gtgaaggtct cccacaggtg tattactttg gaccatgtgg gaaatataat gccatggtgc
      361 tggagctcct tggccctagc ttggaggact tgtttgacct ctgtgaccga acatttactt
      421 tgaagacggt gttaatgata gccatccagc tgctttctcg aatggaatac gtgcactcaa
      481 agaacctcat ttaccgagat gtcaagccag agaacttcct gattggtcga caaggcaata
      541 agaaagagca tgttatacac attatagact ttggactggc caaggaatac attgaccccg
      601 aaaccaaaaa acacatacct tatagggaac acaaaagttt aactggaact gcaagatata
      661 tgtctatcaa cacgcatctt ggcaaagagc aaagccggag agatgatttg gaagccctag
      721 gccatatgtt catgtatttc cttcgaggca gcctccc
//
