LOCUS       sequence-156074-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KC1G1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KC1G1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             276..287
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttagcagccg gatcagcgcg cacgcgtggt accggccggc caccaggtgg cgcgccgcga
       61 tcgcggatcc gttatccact tccaatgtta ttattctcga gttattgcag atgcaccaga
      121 atctacgtga actgacccta ctggagtagg aataggtctc ccaacccaat cataggcata
      181 gtcaaaggtg tagcctttct tttcaaagag gtctgtgaag agggtccgta aatactcata
      241 atcaggtttt tcaaagaagt ccagtcgcct gacatatcga aggtaggttg ccatctcctc
      301 tggaaagttc tcacagagag cttcaatggg agtattcctt ttggtgtcac caattttttg
      361 atatctctct tttaatgtgt cagccttgag tccttgccag gggaggctgc ctcgaaggaa
      421 atacatgaac atatggccta gggcttccaa atcatctctc cggctttgct ctttgccaag
      481 atgcgtgttg atagacatat atcttgcagt tccagttaaa cttttgtgtt ccctataagg
      541 tatgtgtttt ttggtttcgg ggtcaatgta ttccttggcc agtccaaagt ctataatgtg
      601 tataacatgc tctttcttat tgccttgtcg accaatcagg aagttctctg gcttgacatc
      661 tcggtaaatg aggttctttg agtgcacgta ttccattcga gaaagcagct ggatggctat
      721 cattaacacc gtcttcaaag taaatgttcg gtcacagagg tcaaacaagt cctccaagct
      781 agggccaagg agctccagca ccatggcatt atatttcc
//
