LOCUS       sequence-156075-         430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the OK+9MS 
            (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming 
            cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-OK+9MS
FEATURES             Location/Qualifiers
     source          1..430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..90)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 ggtgaagggc gaattgttca tgagtggacc tggattgctt tctacatccc cagccagttt
       61 gagtaaatca aagttaagag tttgtttgac aggagcgaca attttgagat cccctgcacc
      121 agagtttcga agctgttcga gtttgtgttt caactgttct cgtcgtttcc tcaataagtc
      181 cttttcagag gtgagcttgt gctcgtctgc ttgaatggac aggatgtagg cggtggcttt
      241 tttgaggatc actaccttgg gggccttttc gttgttttcc aattcaggga tctggtcacg
      301 cagggcaaaa aagctgcgct tcagctcgtt cctcctctga cgttccaaga cgttgtgtgt
      361 ccgcctcttg tcgttttcct ccgtgtctga ggacctgggg ctggagcact tgcggttgtt
      421 gctgatctgc
//
