LOCUS       sequence-156077-       11405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the OK+9MS 
            (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming 
            cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-OK+9MS
FEATURES             Location/Qualifiers
     source          1..11405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2725..2790
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4255..4314
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5710..5775
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(6815..6839)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6881..7455,7456..7458,7459..7467)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7048..7065)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7275..7294
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7468..8178
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7613..7631)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7881..7900
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8190..8209)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8255..8310
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8309..8328)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   9030..9064
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9214..9232)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9253..9269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9253..9269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9266..9288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9277..9293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9301..9307,9308..9325,9326..9331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9346..9367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9484..9501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9655..10243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9735..9754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10414..11205,11206..11274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11037..11056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11275..11379)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccct
     1621 taaaaagcag gctccacctt ccccatggct ggacacctgg cttcagactt cgccttctca
     1681 cccccaccag gtgggggtga tgggtcagca gggctggagc cgggctgggt ggattctcga
     1741 acctggctaa gcttccaagg gcctccaggt gggcctggaa tcggaccagg ctcagaggta
     1801 ttggggatct ccccatgtcc gcccgcatac gagttctgcg gagggatggc atactgtgga
     1861 cctcaggttg gactgggcct agtcccccaa gttggcgtgg agactttgca gcctgagggc
     1921 caggcaggag cacgagtgga aagcaactca gagggaacct cctctgagcc ctgtgccgac
     1981 cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg
     2041 aaagccctgc agaaggagct agaacagttt gccaagctgc tgaagcagaa gaggatcacc
     2101 ttggggtaca cccaggccga cgtggggctc accctgggcg ttctctttgg aaaggtgttc
     2161 agccagacca ccatctgtcg cttcgaggcc ttgcagctca gccttaagaa catgtgtaag
     2221 ctgcggcccc tgctggagaa gtgggtggag gaagccgaca acaatgagaa ccttcaggag
     2281 atatgcaaat cggagaccct ggtgcaggcc cggaagagaa agcgaactag cattgagaac
     2341 cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc cgaagccctc cctacagcag
     2401 atcactcaca tcgccaatca gcttgggcta gagaaggatg tggttcgagt atggttctgt
     2461 aaccggcgcc agaagggcaa aagatcaagt attgagtatt cccaacgaga agagtatgag
     2521 gctacaggga cacctttccc agggggggct gtatcctttc ctctgccccc aggtccccac
     2581 tttggcaccc caggctatgg aagcccccac ttcaccacac tctactcagt cccttttcct
     2641 gagggcgagg cctttccctc tgttcccgtc actgctctgg gctctcccat gcattcaaac
     2701 ggggatctca aaattgtcgc tcctgtcaaa caaactctta actttgattt actcaaactg
     2761 gctggggatg tagaaagcaa tccaggtcca atgaggcagc cacctggcga gtctgacatg
     2821 gctgtcagcg acgctctgct cccgtccttc tccacgttcg cgtccggccc ggcgggaagg
     2881 gagaagacac tgcgtccagc aggtgccccg actaaccgtt ggcgtgagga actctctcac
     2941 atgaagcgac ttcccccact tcccggccgc ccctacgacc tggcggcgac ggtggccaca
     3001 gacctggaga gtggcggagc tggtgcagct tgcagcagta acaacccggc cctcctagcc
     3061 cggagggaga ccgaggagtt caacgacctc ctggacctag actttatcct ttccaactcg
     3121 ctaacccacc aggaatcggt ggccgccacc gtgaccacct cggcgtcagc ttcatcctcg
     3181 tcttccccag cgagcagcgg ccctgccagc gcgccctcca cctgcagctt cagctatccg
     3241 atccgggccg ggggtgaccc gggcgtggct gccagcaaca caggtggagg gctcctctac
     3301 agccgagaat ctgcgccacc tcccacggcc cccttcaacc tggcggacat caatgacgtg
     3361 agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg agttggaccc agtatacatt
     3421 ccgccacagc agcctcagcc gccaggtggc gggctgatgg gcaagtttgt gctgaaggcg
     3481 tctctgacca cccctggcag cgagtacagc agcccttcgg tcatcagtgt tagcaaagga
     3541 agcccagacg gcagccaccc cgtggtagtg gcgccctaca gcggtggccc gccgcgcatg
     3601 tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg tcagccggtc cctagaggcc
     3661 catttgagcg ctggacccca gctcagcaac ggccaccggc ccaacacaca cgacttcccc
     3721 ctggggcggc agctccccac caggactacc cctacactga gtcccgagga actgctgaac
     3781 agcagggact gtcaccctgg cctgcctctt cccccaggat tccatcccca tccggggccc
     3841 aactaccctc ctttcctgcc agaccagatg cagtcacaag tcccctctct ccattatcaa
     3901 gagctcatgc caccgggttc ctgcctgcca gaggagccca agccaaagag gggaagaagg
     3961 tcgtggcccc ggaaaagaac agccacccac acttgtgact atgcaggctg tggcaaaacc
     4021 tataccaaga gttctcatct caaggcacac ctgcgaactc acacaggcga gaaaccttac
     4081 cactgtgact gggacggctg tgggtggaaa ttcgcccgct ccgatgaact gaccaggcac
     4141 taccgcaaac acacagggca ccggcccttt cagtgccaga agtgcgacag ggccttttcc
     4201 aggtcggacc accttgcctt acacatgaag aggcactttc tcgagggctc gggccagtgt
     4261 actaattatg ctctcttgaa attggctgga gatgttgaga gcaacccagg tcccggcgcg
     4321 ccactggatt tcctttgggc gttggaaacc ccgcagacag ccacgacgat gcccctcaac
     4381 gtgaacttca ccaacaggaa ctatgacctc gactacgact ccgtacagcc ctatttcatc
     4441 tgcgacgagg aagagaattt ctatcaccag caacagcaga gcgagctgca gccgcccgcg
     4501 cccagtgagg atatctggaa gaaattcgag ctgcttccca ccccgcccct gtccccgagc
     4561 cgccgctccg ggctctgctc tccatcctat gttgcggtcg ctacgtcctt ctccccaagg
     4621 gaagacgatg acggcggcgg tggcaacttc tccaccgccg atcagctgga gatgatgacc
     4681 gagttacttg gaggagacat ggtgaaccag agcttcatct gcgatcctga cgacgagacc
     4741 ttcatcaaga acatcatcat ccaggactgt atgtggagcg gtttctcagc cgctgccaag
     4801 ctggtctcgg agaagctggc ctcctaccag gctgcgcgca aagacagcac cagcctgagc
     4861 cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt acctgcagga cctcaccgcc
     4921 gccgcgtccg agtgcattga cccctcagtg gtctttccct acccgctcaa cgacagcagc
     4981 tcgcccaaat cctgtacctc gtccgattcc acggccttct ctccttcctc ggactcgctg
     5041 ctgtcctccg agtcctcccc acgggccagc cctgagcccc tagtgctgca tgaggagaca
     5101 ccgcccacca ccagcagcga ctctgaagaa gagcaagaag atgaggaaga aattgatgtg
     5161 gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg agtcgggctc atctccatcc
     5221 cgaggccaca gcaaacctcc gcacagccca ctggtcctca agaggtgcca cgtctccact
     5281 caccagcaca actacgccgc acccccctcc acaaggaagg actatccagc tgccaagagg
     5341 gccaagttgg acagtggcag ggtcctgaag cagatcagca acaaccgcaa gtgctccagc
     5401 cccaggtcct cagacacgga ggaaaacgac aagaggcgga cacacaacgt cttggaacgt
     5461 cagaggagga acgagctgaa gcgcagcttt tttgccctgc gtgaccagat ccctgaattg
     5521 gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa aagccaccgc ctacatcctg
     5581 tccattcaag cagacgagca caagctcacc tctgaaaagg acttattgag gaaacgacga
     5641 gaacagttga aacacaaact cgaacagctt cgaaactctg gtgcagggga tctcaaaatt
     5701 gtcgctcctg tcaaacaaac tcttaacttt gatttactca aactggctgg ggatgtagaa
     5761 agcaatccag gtccactcat gaacaattcg cccttcacca tgtataacat gatggagacg
     5821 gagctgaagc cgccgggccc gcagcaagct tcggggggcg gcggcggagg aggcaacgcc
     5881 acggcggcgg cgaccggcgg caaccagaag aacagcccgg accgcgtcaa gaggcccatg
     5941 aacgccttca tggtatggtc ccgggggcag cggcgtaaga tggcccagga gaaccccaag
     6001 atgcacaact cggagatcag caagcgcctg ggcgcggagt ggaaactttt gtccgagacc
     6061 gagaagcggc cgttcatcga cgaggccaag cggctgcgcg ctctgcacat gaaggagcac
     6121 ccggattata aataccggcc gcggcggaaa accaagacgc tcatgaagaa ggataagtac
     6181 acgcttcccg gaggcttgct ggcccccggc gggaacagca tggcgagcgg ggttggggtg
     6241 ggcgccggcc tgggtgcggg cgtgaaccag cgcatggaca gctacgcgca catgaacggc
     6301 tggagcaacg gcagctacag catgatgcag gagcagctgg gctacccgca gcacccgggc
     6361 ctcaacgctc acggcgcggc acagatgcaa ccgatgcacc gctacgacgt cagcgccctg
     6421 cagtacaact ccatgaccag ctcgcagacc tacatgaacg gctcgcccac ctacagcatg
     6481 tcctactcgc agcagggcac ccccggtatg gcgctgggct ccatgggctc tgtggtcaag
     6541 tccgaggcca gctccagccc ccccgtggtt acctcttcct cccactccag ggcgccctgc
     6601 caggccgggg acctccggga catgatcagc atgtacctcc ccggcgccga ggtgccggag
     6661 cccgctgcgc ccagtagact gcacatggcc cagcactacc agagcggccc ggtgcccggc
     6721 acggccatta acggcacact gcccctgtcg cacatgtgag ggctggaagg gcgaattgcc
     6781 tgcaggaatt cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg
     6841 atctagctaa aacgcggcct cgaatcgaat tccgcccccc cccctctccc tccccccccc
     6901 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     6961 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     7021 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     7081 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     7141 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     7201 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     7261 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     7321 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     7381 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     7441 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagga taacatggcc
     7501 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     7561 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     7621 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     7681 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     7741 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     7801 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     7861 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     7921 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     7981 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     8041 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     8101 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     8161 gacgagctgt acaagtaaag cggccgcgat ctttttccct ctgccaaaaa ttatggggac
     8221 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     8281 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     8341 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg
     8401 ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     8461 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     8521 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     8581 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga
     8641 cctgcaggaa ttcgatatca agctctagcc aacaagctcg tcatcgcttt gcagaagagc
     8701 agagaggata tgctcatcgt ctaaagaact acccatttta ttatatatta gtcacgatat
     8761 ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat
     8821 ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg
     8881 actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc
     8941 acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt
     9001 tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct
     9061 agggttaaaa aagatttgcg ctttactcga cctaaacttt aaacacgtca tagaatcttc
     9121 gtttgacaaa aaccacattg tggccaagct gtgtgacgcg acgcgcgcta aagaatggca
     9181 aaccaagtcg cgcgaggtac ccagcttttg ttccctttag tgagggttaa ttccgagctt
     9241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10081 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10261 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10321 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11401 acctg
//
