LOCUS       sequence-156079-         418 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Destination donor vector for AAVS1 targeting (puromycin 
            selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-Nst-CAG-DEST
FEATURES             Location/Qualifiers
     source          1..418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(38..44,45..62,63..68))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(93..217)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(284..303)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(365..387)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cctgaaaatc tcgacggatc ctaactcaaa atccacacat tatacgagcc ggaagcataa
       61 agtgtaaagc ctggggtgcc taatgcggcc gccatagtga ctggatatgt tgtgttttac
      121 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
      181 tttacgtttc tcgttcagct tttttgtaca aacttgtgat aacctgcagg atccggcgcg
      241 ccggtaccgc ggccgcgaat tctttgccaa aatgatgaga cagcacaata accagcacgt
      301 tgcccaggag ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc
      361 cgccggtcac acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagcc
//
