LOCUS       sequence-156080-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of DYR1A. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: DYR1A_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca agctcccata agaaggaacg taaggtgtac aacgatggtt
      181 atgacgacga taattacgac tacatcgtta agaacggcga gaagtggatg gatcgctatg
      241 aaattgactc gctgatcggt aaaggctctt tcggtcaggt cgtaaaagcc tatgatcgtg
      301 tggagcaaga atgggtcgca ataaagatca ttaagaacaa gaaggcgttc ttgaaccagg
      361 cacagatcga ggtccgcctg ttggaactga tgaataagca cgataccgag atgaaatact
      421 atatcgtgca tctgaaacgt cacttcatgt ttcgcaatca cctgtgtttg gtattcgaga
      481 tgctgtcata caacctgtat gacctgttgc gtaatactaa cttccgcggc gttagtttga
      541 atctgacacg caaattcgcc caacaaatgt gcaccgcttt gttgtttctg gcgactccag
      601 aattgagcat tattcattgt gatctgaaac ctgagaacat cctgttgtgc aacccgaaac
      661 gctccgcaat caaaattgtc gactttggtt cttcatgtca gctgggccag cgtatctacc
      721 aatacattca gagtcgcttc tatcgttcgc ccgaagtgtt gctgggtatg ccatacgatt
      781 tggccatcga catgtggagc ctgggctgca ttctggtaga aatgcatacg ggagaacctt
      841 tgtt
//
