LOCUS       sequence-156083-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK01. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK01_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca atggcggcgg cggcggcggc gggcgcgggc ccggagatgg
      181 tccgcgggca ggtgttcgac gtggggccgc gctacaccaa cctctcgtac atcggcgagg
      241 gcgcctacgg catggtgtgc tctgcttatg ataatgtcaa caaagttcga gtagctatca
      301 agaaaatcag cccctttgag caccagacct actgccagag aaccctgagg gagataaaaa
      361 tcttactgcg cttcagacat gagaacatca ttggaatcaa tgacattatt cgagcaccaa
      421 ccatcgagca aatgaaagat gtatatatag tacaggacct catggaaaca gatctttaca
      481 agctcttgaa gacacaacac ctcagcaatg accatatctg ctattttctc taccagatcc
      541 tcagagggtt aaaatatatc cattcagcta acgttctgca ccgtgacctc aagccttcca
      601 acctgctgct caacaccacc tgtgatctca agatctgtga ctttggcctg gcccgtgttg
      661 cagatccaga ccatgatcac acagggttcc tgacagaata tgtggccaca cgttggtaca
      721 gggctccaga aattatgttg aattccaagg gctacaccaa gtccattgat atttggtctg
      781 taggctgcat tctggcagaa atgctttcta acaggcccat ctttccagg
//
