LOCUS       sequence-156084-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK01. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK01_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(94..129)
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 ttagcagccg gatcagcgcg cacgcgtggt accggccggc caccaggtgg cgcgccgcga
       61 tcgcggatcc gttatccact tccaatgtta ttaagatctg tatcctggct ggaatctagc
      121 agtctcttca aaaattagtt ctttgagctt ttccttaggc aagtcatcca attccatgtc
      181 gaacttgaat ggtgcttcgg cgatgggctc gtcactcggg tcgtaatact gctccagata
      241 tgggtgggcc agagcctgtt ctacttcaat cctcttgtgt gggttgaatg tcaacatttt
      301 gtccaataag tccagagctt tggagtcagc atttgggaac agcctgttcc atggcacctt
      361 atttttgtgt ggaagagaaa gcaaatagtt cctagctttt aaatttatta tacaattcag
      421 gtcttcttgt gatggggatc caagaatacc caaaatgtgg ttcagctggt caagataatg
      481 cttccctgga aagatgggcc tgttagaaag catttctgcc agaatgcagc ctacagacca
      541 aatatcaatg gacttggtgt agcccttgga attcaacata atttctggag ccctgtacca
      601 acgtgtggcc acatattctg tcaggaaccc tgtgtgatca tggtctggat ctgcaacacg
      661 ggccaggcca aagtcacaga tcttgagatc acaggtggtg ttgagcagca ggttggaagg
      721 cttgaggtca cggtgcagaa cgttagctga atggatatat tttaaccctc tgaggatctg
      781 gtagagaaaa tagcagatat ggtcattgct gaggtgttgt gtcttcaaga gcttgtaaag
      841 atctgtttc
//
