LOCUS       sequence-156089-         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for amplifying the attL1-em7-kanr-attL2-pA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Zhao L, Ng ET, Koopman P
  TITLE     A piggyBac transposon- and gateway-enhanced system for efficient BAC
            transgenesis.
  JOURNAL   Dev Dyn. 2014 Jun 12. doi: 10.1002/dvdy.24153.
  PUBMED    24924516
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2014 Jun 12. doi: 10.1002/dvdy.24153."
COMMENT     Sequence Label: pBSWt1-Dest
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(62..109)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    complement(124..248)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acctgcgtgc aatccatctt gttcaatcat gatctgcctc ctgggtttag ttcctcacct
       61 tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaacg aattcgcggc
      121 cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc ttttttgtac
      241 aaacttgtga tggccgcttg ctgtctgcag cccgggggat ctgaggtgcg gctcggatgc
      301 ggacggttct ccgctcctgc gatcctgcgg attctcggcg ctggacttgg cccaggcgct
      361 ccgggggcct cggtccccag gttgctccgg ctgctggacg cacccgggct cccggcggag
      421 cgtgtgctga gacgccggct ccgggacaca cgtggaagcc ggctcctgcg acaggaggaa
      481 gtccaggatc gcggcgagga ggcggcgggc ccgggttcct gggctgccgt cctctctctg
      541 ggtgggtggg tgaatgagtg ggtgggaggg agggc
//
