LOCUS       sequence-156093-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of VRK2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: VRK2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca ccatttccag aaggcaaggt tctggatgat atggaaggca
      181 atcagtgggt actgggcaag aagattggct ctggaggatt tggattgata tatttagctt
      241 tccccacaaa taaaccagag aaagatgcaa gacatgtagt aaaagtggaa tatcaagaaa
      301 atggcccgtt attttcagaa cttaaatttt atcagagagt tgcaaaaaaa gactgtatca
      361 aaaagtggat agaacgcaaa caacttgatt atttaggaat tcctctgttt tatggatctg
      421 gtctgaccga attcaaggga agaagttaca gatttatggt aatggaaaga ctaggaatag
      481 atttacagaa gatctcaggc cagaatggta cctttaaaaa gtcaactgtc ctgcaattag
      541 gtatccgaat gttggatgta ctggaatata tacatgaaaa tgaatatgtt catggtgata
      601 taaaagcagc aaatctactt ttgggttaca aaaatccaga ccaggtttat cttgcagatt
      661 atggactttc ctacagatat tgtcccaatg ggaaccacaa acagtatcag gaaaatccta
      721 gaaaaggcca taatgggaca atagagttta ccagcttgga tgcccacaag ggagtagcct
      781 tgtccagacg aagtgacgtt gagatcctcg gctactgcat gctgcggtgg ttgtgtgg
//
