LOCUS       sequence-156097-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for amplifying the attL1-em7-kanr-attL2-pA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Zhao L, Ng ET, Koopman P
  TITLE     A piggyBac transposon- and gateway-enhanced system for efficient BAC
            transgenesis.
  JOURNAL   Dev Dyn. 2014 Jun 12. doi: 10.1002/dvdy.24153.
  PUBMED    24924516
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2014 Jun 12. doi: 10.1002/dvdy.24153."
COMMENT     Sequence Label: pBSWt1-Dest
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(137..261)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(311..330)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     365..384
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             531..548
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
       61 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
      121 aagcttgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
      181 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
      241 agctttcttg tacaaagtgg tgatggccgc tgactcgagt ttattgcagc ttataatggt
      301 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      361 agttgtggtt tgtccaaact catcaatgta tcttaattaa ttaacgccgc ttctcccctt
      421 gtctgttcct tcctgatgcc agttcttact cagcagcctt ccaagggagt ctgaaagtag
      481 cggacaggga ggagtgggct ctgtcaaatg cgagtgacaa cgcgacaacg caccaccacc
      541 accaccactt acacattact gagcgcaacc ccagagtccg atagttcgta ggacgcggga
      601 ggacatctgg ggccccgggg tgtcctagag atttcggaac tccacgggag gaggaagagg
      661 aggttggctg taacgtgtga ctctacaccg ggggaaagtc tgatgcataa ggcctcacca
      721 ggcgttgact cttaaaacgt ctagggtaga ccaagatgag gactccgagt ggtgctgtgg
      781 tttagggaca tcccccaccc cccgcccccg tgctgcctct gacaagcccg aagagcc
//
