LOCUS       sequence-156099-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the EB-C5 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-EB-C5
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(321..374)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gattcctggt gggttagcga gttggaaagg ataaagtcta ggtccaggag gtcgttgaac
       61 tcctcggtct ccctccgggc taggagggcc gggttgttac tgctgcaagc tgcaccagct
      121 ccgccactct ccaggtctgt ggccaccgtc gccgccaggt cgtaggggcg gccgggaagt
      181 gggggaagtc gcttcatgtg agagagttcc tcacgccaac ggttagtcgg ggcacctgct
      241 ggacgcagtg tcttctccct tcccgccggg ccggacgcga acgtggagaa ggacgggagc
      301 agagcgtcgc tgacagccat tgggccagga ttctcctcca cgtcaccgca tgttagaaga
      361 cttcctctgc cctctcctcc ggacccgccg cccatgtgcg acaggggcag tgtgccgtta
      421 atggccgtgc cgggcaccgg gccgctctgg tagtgctggg ccatgtgcag tctactgggc
      481 gcagcgggct ccggcacctc ggcgccgggg aggtacatgc tgatcatgtc ccggaggtcc
      541 ccggcctggc agggcgccct ggagtgggag gaagaggtaa ccacgggggg gctggagctg
      601 gcctcggact tgaccacaga gcccatggag cccagcgcca taccgggggt gccctgctgc
      661 gagtaggaca tgctgtaggt gggcgagccg ttcatgtagg tctgcgagct ggtcatggag
      721 ttgtactgca gggcgctgac gtcgtagcgg tgcatcggtt gcatctgtgc cgcgccgtga
      781 gcgttgaggc ccgggtgctg cg
//
