LOCUS       sequence-156100-       11905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the EB-C5 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-EB-C5
FEATURES             Location/Qualifiers
     source          1..11905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2699..2752
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3728..3781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6617..6670
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(7315..7339)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(7381..7955,7956..7958,7959..7967)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7548..7565)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7775..7794
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7968..8678
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8113..8131)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8381..8400
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8690..8709)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8755..8810
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8809..8828)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   9530..9564
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9714..9732)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9753..9769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9753..9769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9766..9788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9777..9793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9801..9807,9808..9825,9826..9831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9846..9867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9984..10001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10155..10743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10235..10254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10914..11705,11706..11774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11537..11556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11775..11879)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcagatc
     1621 caccatggct ggacacctgg cttcagactt cgccttctca cccccaccag gtgggggtga
     1681 tgggtcagca gggctggagc cgggctgggt ggattctcga acctggctaa gcttccaagg
     1741 gcctccaggt gggcctggaa tcggaccagg ctcagaggta ttggggatct ccccatgtcc
     1801 gcccgcatac gagttctgcg gagggatggc atactgtgga cctcaggttg gactgggcct
     1861 agtcccccaa gttggcgtgg agactttgca gcctgagggc caggcaggag cacgagtgga
     1921 aagcaactca gagggaacct cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt
     1981 ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg aaagccctgc agaaggagct
     2041 agaacagttt gccaagctgc tgaagcagaa gaggatcacc ttggggtaca cccaggccga
     2101 cgtggggctc accctgggcg ttctctttgg aaaggtgttc agccagacca ccatctgtcg
     2161 cttcgaggcc ttgcagctca gccttaagaa catgtgtaag ctgcggcccc tgctggagaa
     2221 gtgggtggag gaagccgaca acaatgagaa ccttcaggag atatgcaaat cggagaccct
     2281 ggtgcaggcc cggaagagaa agcgaactag cattgagaac cgtgtgaggt ggagtctgga
     2341 gaccatgttt ctgaagtgcc cgaagccctc cctacagcag atcactcaca tcgccaatca
     2401 gcttgggcta gagaaggatg tggttcgagt atggttctgt aaccggcgcc agaagggcaa
     2461 aagatcaagt attgagtatt cccaacgaga agagtatgag gctacaggga cacctttccc
     2521 agggggggct gtatcctttc ctctgccccc aggtccccac tttggcaccc caggctatgg
     2581 aagcccccac ttcaccacac tctactcagt cccttttcct gagggcgagg cctttccctc
     2641 tgttcccgtc actgctctgg gctctcccat gcattcaaac ggcggcgggt ccggaggaga
     2701 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcctggcc caatgtataa
     2761 catgatggag acggagctga agccgccggg cccgcagcaa gcttcggggg gcggcggcgg
     2821 aggaggcaac gccacggcgg cggcgaccgg cggcaaccag aagaacagcc cggaccgcgt
     2881 caagaggccc atgaacgcct tcatggtatg gtcccggggg cagcggcgta agatggccca
     2941 ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgcgg agtggaaact
     3001 tttgtccgag accgagaagc ggccgttcat cgacgaggcc aagcggctgc gcgctctgca
     3061 catgaaggag cacccggatt ataaataccg gccgcggcgg aaaaccaaga cgctcatgaa
     3121 gaaggataag tacacgcttc ccggaggctt gctggccccc ggcgggaaca gcatggcgag
     3181 cggggttggg gtgggcgccg gcctgggtgc gggcgtgaac cagcgcatgg acagctacgc
     3241 gcacatgaac ggctggagca acggcagcta cagcatgatg caggagcagc tgggctaccc
     3301 gcagcacccg ggcctcaacg ctcacggcgc ggcacagatg caaccgatgc accgctacga
     3361 cgtcagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc
     3421 cacctacagc atgtcctact cgcagcaggg cacccccggt atggcgctgg gctccatggg
     3481 ctctgtggtc aagtccgagg ccagctccag cccccccgtg gttacctctt cctcccactc
     3541 cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtacc tccccggcgc
     3601 cgaggtgccg gagcccgctg cgcccagtag actgcacatg gcccagcact accagagcgg
     3661 cccggtgccc ggcacggcca ttaacggcac actgcccctg tcgcacatgg gcggcgggtc
     3721 cggaggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcctggccc
     3781 aatggctgtc agcgacgctc tgctcccgtc cttctccacg ttcgcgtccg gcccggcggg
     3841 aagggagaag acactgcgtc cagcaggtgc cccgactaac cgttggcgtg aggaactctc
     3901 tcacatgaag cgacttcccc cacttcccgg ccgcccctac gacctggcgg cgacggtggc
     3961 cacagacctg gagagtggcg gagctggtgc agcttgcagc agtaacaacc cggccctcct
     4021 agcccggagg gagaccgagg agttcaacga cctcctggac ctagacttta tcctttccaa
     4081 ctcgctaacc caccaggaat cggtggccgc caccgtgacc acctcggcgt cagcttcatc
     4141 ctcgtcttcc ccggcgagca gcggccctgc cagcgcgccc tccacctgca gcttcagcta
     4201 tccgatccgg gccgggggtg acccgggcgt ggctgccaga aacacaggtg gagggctcct
     4261 ctacagccga gaatctgcgc cacctcccac ggcccccttc aacctggggg acatcaatga
     4321 cgtgagcccc tcgggcggct tcgtggctga gctcctgcgg ccggagttgg acccagtata
     4381 cattccgcca cagcagcctc agccgccagg tggggggctg atgggcaagt ttgtgctgaa
     4441 ggcgtctctg accacccctg gcagcgagta cagcagccct tcggtcatca gtgttagcaa
     4501 aggaagccca gacggcagcc accccgtggt agtggcgccc tacagcggtg gcccgccgcg
     4561 catgtgcccc aagattaagc aagaggcggt cccgtcctgc acggtcagcc ggtccctaga
     4621 ggcccatttg agcgctggac cccagctcag caacggccac cggcccaaca cacacgactt
     4681 ccccctgggg cggcagctcc ccaccaggac tacccctaca ctgagtcccg aggaactgct
     4741 gaacagcagg gactgtcacc ctggcctgcc tcttccccca ggattccatc cccatccggg
     4801 ggccaactac cctcctttcc tgccagacca gatgcagtca caagtcccct ctctccatta
     4861 tcaagagctc atgccaccgg gttcctgcct gccagaggag cccaagccaa agaggggaag
     4921 aaggtcgtgg ccccggaaaa gaacagccac ccacacttgt gactatgcag gctgtggcaa
     4981 aacctatacc aagagttctc atctcaaggc acacctgcga actcacacag gcgagaaacc
     5041 ttaccactgt gactgggacg gctgtgggtg gaaattcgcc cgctccgatg aactgaccag
     5101 gcactaccgc aaacacacag ggcaccggcc ctttcagtgc cagaagtgtg acagggcctt
     5161 ttccaggtcg gaccaccttg ccttacacat gaagaggcac tttggcagcg gaaaacagct
     5221 gttgaatttt gaccttctca agttggcggg agacgtggag tccaacccag ggcccgtcga
     5281 gatgcccctc aacgtgaact tcaccaacag gaactatgac ctcgactacg actccgtaca
     5341 gccctatttc atctgcgacg aggaagagaa tttctatcac cagcaacagc agagcgagct
     5401 gcagccgccc gcgcccagtg aggatatctg gaagaaattc gagctgcttc ccaccccgcc
     5461 cctgtccccg agccgccgct ccgggctctg ctctccatcc tatgttgcgg tcgctacgtc
     5521 cttctcccca agggaagacg atgacggcgg cggtggcaac ttctccaccg ccgatcagct
     5581 ggagatgatg accgagttac ttggaggaga catggtgaac cagagcttca tctgcgatcc
     5641 tgacgacgag accttcatca agaacatcat catccaggac tgtatgtgga gcggtttctc
     5701 agccgctgcc aagctggtct cggagaagct ggcctcctac caggctgcgc gcaaagacag
     5761 caccagcctg agccccgccc gcgggcacag cgtctgctcc acctccagcc tgtacctgca
     5821 ggacctcacc gccgccgcgt ccgagtgcat tgacccctca gtggtctttc cctacccgct
     5881 caacgacagc agctcgccca aatcctgtac ctcgtccgat tccacggcct tctctccttc
     5941 ctcggactcg ctgctgtcct ccgagtcctc cccacgggcc agccctgagc ccctagtgct
     6001 gcatgaggag acaccgccca ccaccagcag cgactctgaa gaagagcaag aagatgagga
     6061 agaaattgat gtggtgtctg tggagaagag gcaaacccct gccaagaggt cggagtcggg
     6121 ctcatctcca tcccgaggcc acagcaaacc tccgcacagc ccactggtcc tcaagaggtg
     6181 ccacgtctcc actcaccagc acaactacgc cgcacccccc tccacaagga aggactatcc
     6241 agctgccaag agggccaagt tggacagtgg cagggtcctg aagcagatca gcaacaaccg
     6301 caagtgctcc agccccaggt cctcagacac ggaggaaaac gacaagaggc ggacacacaa
     6361 cgtcttggaa cgtcagagga ggaacgagct gaagcgcagc ttttttgccc tgcgtgacca
     6421 gatccctgaa ttggaaaaca acgaaaaggc ccccaaggta gtgatcctca aaaaagccac
     6481 cgcctacatc ctgtccattc aagcagacga gcacaagctc acctctgaaa aggacttatt
     6541 gaggaaacga cgagaacagt tgaaacacaa actcgaacag cttcgaaact ctggtgcagg
     6601 cggcgggtcc ggaggagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     6661 tcctggccca atgggctcgg tgtccaacca gcagtttgca ggtggctgcg ccaaggcagc
     6721 ggagaaggcg ccagaggagg cgccgcctga cgcggcccga gcggcagacg agccgcagct
     6781 gctgcacggg gccggcatct gtaagtggtt caacgtgcgc atggggttcg gcttcctgtc
     6841 tatgaccgcc cgcgccgggg tcgcgctcga ccccccggtg gacgtctttg tgcaccagag
     6901 caagctgcac atggaagggt tccgaagcct caaggagggt gaggcggtgg agttcacctt
     6961 taagaagtct gccaagggtc tggaatccat ccgtgtcact ggccctggtg gtgtgttctg
     7021 tattggaagt gagcggcggc caaaggggaa gaacatgcag aagcgaagat ccaaaggaga
     7081 caggtgctac aactgcggtg ggctagacca tcatgccaag gaatgcaagc tgccacccca
     7141 gcccaagaag tgccactttt gccaaagcat caaccatatg gtggcctcgt gtccactgaa
     7201 ggcccagcag ggccccagtt ctcagggaaa gcctgcctac ttccgggagg aagaggaaga
     7261 gatccacagc cctgccctgc tcccagaagc ccagaattga ctcgagatat ctagacccag
     7321 ctttcttgta caaagtggtg atctagctaa aacgcggcct cgaatcgaat tccgcccccc
     7381 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     7441 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     7501 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     7561 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     7621 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     7681 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     7741 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7801 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     7861 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     7921 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
     7981 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     8041 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     8101 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     8161 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     8221 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     8281 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     8341 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     8401 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     8461 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     8521 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     8581 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     8641 gccactccac cggcggcatg gacgagctgt acaagtaaag cggccgcgat ctttttccct
     8701 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     8761 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     8821 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     8881 catatgccat atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg
     8941 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     9001 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     9061 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     9121 ctcttatgaa gatccctcga cctgcaggaa ttcgatatca agctctagcc aacaagctcg
     9181 tcatcgcttt gcagaagagc agagaggata tgctcatcgt ctaaagaact acccatttta
     9241 ttatatatta gtcacgatat ctataacaag aaaatatata tataataagt tatcacgtaa
     9301 gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa
     9361 gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac
     9421 cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac
     9481 agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat
     9541 tttacgcaga ctatctttct agggttaaaa aagatttgcg ctttactcga cctaaacttt
     9601 aaacacgtca tagaatcttc gtttgacaaa aaccacattg tggccaagct gtgtgacgcg
     9661 acgcgcgcta aagaatggca aaccaagtcg cgcgaggtac ccagcttttg ttccctttag
     9721 tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9841 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9901 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9961 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    10021 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10081 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    10141 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10201 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10261 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10321 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10381 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10441 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10501 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10561 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    10621 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10681 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10741 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10801 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10861 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10921 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10981 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    11041 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    11101 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    11161 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    11221 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11281 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11341 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11401 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    11461 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    11521 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11581 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11641 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11701 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11761 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11821 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11881 acatttcccc gaaaagtgcc acctg
//
