LOCUS       sequence-156101-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KCC2A. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KCC2A_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca taccagctct tcgaggaatt gggcaaggga gccttctcgg
      181 tggtgcgaag gtgtgtgaag gtgctggctg gccaggagta tgctgccaag atcatcaaca
      241 caaagaagct gtcagccaga gaccatcaga agctggagcg tgaagcccgc atctgccgcc
      301 tgctgaagca ccccaacatc gtccgactac atgacagcat ctcagaggag ggacaccact
      361 acctgatctt cgacctggtc actggtgggg aactgtttga agatatcgtg gcccgggagt
      421 attacagtga ggcggatgcc agtcactgta tccagcagat cctggaggct gtgctgcact
      481 gccaccagat gggggtggtg caccgggacc tgaagcctga gaatctgttg ctggcctcca
      541 agctcaaggg tgccgcagtg aagctggcag actttggcct ggccatagag gtggaggggg
      601 agcagcaggc atggtttggg tttgcaggga ctcctggata tctctcccca gaagtgctgc
      661 ggaaggaccc gtacgggaag cctgtggacc tgtgggcttg tggggtcatc ctgtacatcc
      721 tgctggttgg gtaccccccg ttctgggatg aggaccagca ccgcctgtac cagcagatca
      781 aagccggcgc ctatgatttc ccatcgccgg aatgg
//
